White-nose syndrome pathology grading in Nearctic and
Palearctic bats

J 2017

White-nose syndrome pathology grading in Nearctic and Palearctic bats

PIKULA, J., S.K. AMELON, H. BANDOUCHOVA, Tomáš BARTONIČKA, Hana BERKOVÁ et. al.

Basic information

Original name

White-nose syndrome pathology grading in Nearctic and Palearctic bats

Authors

Edition

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.766

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pone.0180435

UT WoS

000406768200008

Keywords in English

PSEUDOGYMNOASCUS-DESTRUCTANS; GEOMYCES-DESTRUCTANS; MYOTIS-LUCIFUGUS; INFECTION; MORTALITY; SUGGESTS; WINGS

Abstract

V originále

While white-nose syndrome (WNS) has decimated hibernating bat populations in the Nearctic, species from the Palearctic appear to cope better with the fungal skin infection causing WNS. This has encouraged multiple hypotheses on the mechanisms leading to differential survival of species exposed to the same pathogen. To facilitate intercontinental comparisons, we proposed a novel pathogenesis-based grading scheme consistent with WNS diagnosis histopathology criteria. UV light-guided collection was used to obtain single biopsies from Nearctic and Palearctic bat wing membranes non-lethally. The proposed scheme scores eleven grades associated with WNS on histopathology. Given weights reflective of grade severity, the sum of findings from an individual results in weighted cumulative WNS pathology score. The probability of finding fungal skin colonisation and single, multiple or confluent cupping erosions increased with increase in Pseudogymnoascus destructans load. Increasing fungal load mimicked progression of skin infection from epidermal surface colonisation to deep dermal invasion. Similarly, the number of UV-fluorescent lesions increased with increasing weighted cumulative WNS pathology score, demonstrating congruence between WNS-associated tissue damage and extent of UV fluorescence. In a case report, we demonstrated that UV-fluorescence disappears within two weeks of euthermy. Change in fluorescence was coupled with a reduction in weighted cumulative WNS pathology score, whereby both methods lost diagnostic utility. While weighted cumulative WNS pathology scores were greater in the Nearctic than Palearctic, values for Nearctic bats were within the range of those for Palearctic species. Accumulation of wing damage probably influences mortality in affected bats, as demonstrated by a fatal case of Myotis daubentonii with natural WNS infection and healing in Myotis myotis. The proposed semi-quantitative pathology score provided good agreement between experienced raters, showing it to be a powerful and widely applicable tool for defining WNS severity. A

Links

GAP506/12/1064, research and development project

Name: Adaptace netopýrů na plísňové onemocnění geomykózu

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PIKULA, J., S.K. AMELON, H. BANDOUCHOVA, Tomáš BARTONIČKA, Hana BERKOVÁ, J. BRICHTA, S. HOOPER, T. KOKUREWICZ, M. KOLARIK, B. KOLLNER, V. KOVACOVA, P. LINHART, V. PIACEK, G.G. TURNER, Jan ZUKAL and Natália MARTÍNKOVÁ. White-nose syndrome pathology grading in Nearctic and Palearctic bats. Plos one. San Francisco: Public Library of Science, 2017, vol. 12, No 8, p. 1-21. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0180435.

@article{1394381,
   author = {Pikula, J. and Amelon, S.K. and Bandouchova, H. and Bartonička, Tomáš and Berková, Hana and Brichta, J. and Hooper, S. and Kokurewicz, T. and Kolarik, M. and Kollner, B. and Kovacova, V. and Linhart, P. and Piacek, V. and Turner, G.G. and Zukal, Jan and Martínková, Natália},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0180435},
   keywords = {PSEUDOGYMNOASCUS-DESTRUCTANS; GEOMYCES-DESTRUCTANS; MYOTIS-LUCIFUGUS; INFECTION; MORTALITY; SUGGESTS; WINGS},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   note = {Nepřenášet pro RIV, jedná se o duplicitní záznam (2. záznam už je označen pro RIV), který nelze smazat z ISu, protože je na u něj odkaz na téma DP.},
   title = {White-nose syndrome pathology grading in Nearctic and Palearctic bats},
   url = {http://dx.doi.org/10.1371/journal.pone.0180435},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1394381
AU  - Pikula, J. - Amelon, S.K. - Bandouchova, H. - Bartonička, Tomáš - Berková, Hana - Brichta, J. - Hooper, S. - Kokurewicz, T. - Kolarik, M. - Kollner, B. - Kovacova, V. - Linhart, P. - Piacek, V. - Turner, G.G. - Zukal, Jan - Martínková, Natália
PY  - 2017
TI  - White-nose syndrome pathology grading in Nearctic and Palearctic bats
JF  - Plos one
VL  - 12
IS  - 8
SP  - 1-21
EP  - 1-21
PB  - Public Library of Science
SN  - 19326203
N1  - Nepřenášet pro RIV, jedná se o duplicitní záznam (2. záznam už je označen pro RIV), který nelze smazat z ISu, protože je na u něj odkaz na téma DP.
KW  - PSEUDOGYMNOASCUS-DESTRUCTANS
KW  - GEOMYCES-DESTRUCTANS
KW  - MYOTIS-LUCIFUGUS
KW  - INFECTION
KW  - MORTALITY
KW  - SUGGESTS
KW  - WINGS
UR  - http://dx.doi.org/10.1371/journal.pone.0180435
L2  - http://dx.doi.org/10.1371/journal.pone.0180435
N2  - While white-nose syndrome (WNS) has decimated hibernating bat populations in the Nearctic, species from the Palearctic appear to cope better with the fungal skin infection causing WNS. This has encouraged multiple hypotheses on the mechanisms leading to differential survival of species exposed to the same pathogen. To facilitate intercontinental comparisons, we proposed a novel pathogenesis-based grading scheme consistent with WNS diagnosis histopathology criteria. UV light-guided collection was used to obtain single biopsies from Nearctic and Palearctic bat wing membranes non-lethally. The proposed scheme scores eleven grades associated with WNS on histopathology. Given weights reflective of grade severity, the sum of findings from an individual results in weighted cumulative WNS pathology score. The probability of finding fungal skin colonisation and single, multiple or confluent cupping erosions increased with increase in Pseudogymnoascus destructans load. Increasing fungal load mimicked progression of skin infection from epidermal surface colonisation to deep dermal invasion. Similarly, the number of UV-fluorescent lesions increased with increasing weighted cumulative WNS pathology score, demonstrating congruence between WNS-associated tissue damage and extent of UV fluorescence. In a case report, we demonstrated that UV-fluorescence disappears within two weeks of euthermy. Change in fluorescence was coupled with a reduction in weighted cumulative WNS pathology score, whereby both methods lost diagnostic utility. While weighted cumulative WNS pathology scores were greater in the Nearctic than Palearctic, values for Nearctic bats were within the range of those for Palearctic species. Accumulation of wing damage probably influences mortality in affected bats, as demonstrated by a fatal case of Myotis daubentonii with natural WNS infection and healing in Myotis myotis. The proposed semi-quantitative pathology score provided good agreement between experienced raters, showing it to be a powerful and widely applicable tool for defining WNS severity. A
ER  -

PIKULA, J., S.K. AMELON, H. BANDOUCHOVA, Tomáš BARTONIČKA, Hana BERKOVÁ, J. BRICHTA, S. HOOPER, T. KOKUREWICZ, M. KOLARIK, B. KOLLNER, V. KOVACOVA, P. LINHART, V. PIACEK, G.G. TURNER, Jan ZUKAL and Natália MARTÍNKOVÁ. White-nose syndrome pathology grading in Nearctic and Palearctic bats. \textit{Plos one}. San Francisco: Public Library of Science, 2017, vol.~12, No~8, p.~1-21. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0180435.

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