HEGEROVA, Dagmar, Radek VESELÝ, Kristyna CIHALOVA, Pavel KOPEL, Vedran MILOSAVLJEVIC, Zbynek HEGER, David HYNEK, Roman GURÁŇ, Markéta VACULOVIČOVÁ, Pavel SEDLACEK and Vojtěch ADAM. Antimicrobial Agent Based on Selenium Nanoparticles and Carboxymethyl Cellulose for the Treatment of Bacterial Infections. Journal of Biomedical Nanotechnology. American Scientific Publishers, 2017, vol. 13, No 7, p. 767-777. ISSN 1550-7033. Available from: https://dx.doi.org/10.1166/jbn.2017.2384.
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Basic information
Original name Antimicrobial Agent Based on Selenium Nanoparticles and Carboxymethyl Cellulose for the Treatment of Bacterial Infections
Authors HEGEROVA, Dagmar, Radek VESELÝ (203 Czech Republic, belonging to the institution), Kristyna CIHALOVA, Pavel KOPEL, Vedran MILOSAVLJEVIC, Zbynek HEGER, David HYNEK, Roman GURÁŇ (203 Czech Republic), Markéta VACULOVIČOVÁ (203 Czech Republic), Pavel SEDLACEK and Vojtěch ADAM (203 Czech Republic, guarantor).
Edition Journal of Biomedical Nanotechnology, American Scientific Publishers, 2017, 1550-7033.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10302 Condensed matter physics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.068
RIV identification code RIV/00216224:14110/17:00108765
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1166/jbn.2017.2384
UT WoS 000414129200003
Keywords in English Antimicrobial; Clinical isolates; Composite; Inhibition; Synthesis
Tags International impact, Reviewed
Changed by Changed by: Mgr. Roman Guráň, učo 270205. Changed: 27/10/2021 11:53.
Abstract
Our main objective was to analyse and study the effects of the synthesized composite based on selenium nanoparticles and carboxymethyl cellulose (Cekol), hereinafter denoted as SeNPs-Cekol. Firstly, physico-chemical properties of SeNPs-Cekol were characterized in greater detail (size of nanoparticles-from 50 to 150 nm; content of selenium-278 ppm; pH of composite-5.4-5.6; density-990-1010 kg/m(3)), together with assessment of its stability. In addition, the toxicity and mutagenicity on prokaryotic and eukaryotic cells was successfully evaluated. All of the tested bacterial strains were isolated from wound swabs of infectious patients (n = 300) and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). These strains were consequently exposed to SeNPs-Cekol composite. Almost all of the bacterial strains (n = 63) exhibited inhibition zones larger than 5 mm (limit for sensitivity to antibiotics) after the application of the SeNPs-Cekol (300 mu M). Furthermore, in some tested strains (n = 8 for gram positive (G(+)); n = 4 for gram negative (G(-))) even the inhibition zones larger than 12 mm (limit value for very sensitive bacteria to antibiotics) were observed. Overall, the effects of the composite were higher for the G(+) bacteria in comparison with G(-)bacteria, which are generally more resistant to antimicrobial agents, due to their cell wall structure. Further, we found that mutagenicity of the SeNPs-Cekol was found to be negligible. Even, non-target toxicity tests towards eukaryotic cells did not show any significant inhibition of the cells growth compared to the control. Therefore, it can be concluded that SeNPs-Cekol could be considered to have a potential in treatment of bacterial infections.
Links
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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