| GROCHALOVÁ, Martina, Hana KONEČNÁ, Karel STEJSKAL, David POTĚŠIL, Danuše FRIDRICHOVÁ, Eva SRBOVÁ, Kateřina ORNEROVÁ and Zbyněk ZDRÁHAL. Deep coverage of the beer proteome. Journal of Proteomics. Amsterdam: ELSEVIER SCIENCE BV, 2017, vol. 162, JUN, p. 119-124. ISSN 1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2017.05.001. |
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@article{1394837,
author = {Grochalová, Martina and Konečná, Hana and Stejskal, Karel and Potěšil, David and Fridrichová, Danuše and Srbová, Eva and Ornerová, Kateřina and Zdráhal, Zbyněk},
article_location = {Amsterdam},
article_number = {JUN},
doi = {http://dx.doi.org/10.1016/j.jprot.2017.05.001},
keywords = {Beer proteome; Gluten proteins; IPG-IEF; LC-MS/MS},
language = {eng},
issn = {1874-3919},
journal = {Journal of Proteomics},
title = {Deep coverage of the beer proteome},
url = {https://www.sciencedirect.com/science/article/pii/S1874391917301501?via%3Dihub},
volume = {162},
year = {2017}
}
TY - JOUR
ID - 1394837
AU - Grochalová, Martina - Konečná, Hana - Stejskal, Karel - Potěšil, David - Fridrichová, Danuše - Srbová, Eva - Ornerová, Kateřina - Zdráhal, Zbyněk
PY - 2017
TI - Deep coverage of the beer proteome
JF - Journal of Proteomics
VL - 162
IS - JUN
SP - 119-124
EP - 119-124
PB - ELSEVIER SCIENCE BV
SN - 18743919
KW - Beer proteome
KW - Gluten proteins
KW - IPG-IEF
KW - LC-MS/MS
UR - https://www.sciencedirect.com/science/article/pii/S1874391917301501?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S1874391917301501?via%3Dihub
N2 - We adopted an approach based on peptide immobilized pH gradient-isoelectric focusing (IPG-IEF) separation, coupled with LC-MS/MS, in order to maximize coverage of the beer proteome. A lager beer brewed using traditional Czech technology was degassed, desalted and digested. Tryptic peptides were separated by isoelectric focusing on an immobilized pH gradient strip and, after separation, the gel strip was divided into seven equally sized parts. Peptides extracted from gel fractions were analyzed by LC-MS/MS. This approach resulted in a three-fold increase in the number of proteins identified (over 1700) when compared to analysis of unfractionated beer processed by a filter-aided sample preparation (FASP). Over 1900 protein groups (PGs) in total were identified by both approaches.
ER -
GROCHALOVÁ, Martina, Hana KONEČNÁ, Karel STEJSKAL, David POTĚŠIL, Danuše FRIDRICHOVÁ, Eva SRBOVÁ, Kateřina ORNEROVÁ and Zbyněk ZDRÁHAL. Deep coverage of the beer proteome. \textit{Journal of Proteomics}. Amsterdam: ELSEVIER SCIENCE BV, 2017, vol.~162, JUN, p.~119-124. ISSN~1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2017.05.001.
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