Detailed Information on Publication Record
2017
Epistatic and allelic interactions control expression of ribosomal RNA gene clusters in Arabidopsis thaliana
RABANAL, Fernando A., Terezie MANDÁKOVÁ, Luz M. SOTO-JIMENEZ, Robert GREENHALGH, David L. PARROTT et. al.Basic information
Original name
Epistatic and allelic interactions control expression of ribosomal RNA gene clusters in Arabidopsis thaliana
Authors
RABANAL, Fernando A. (40 Austria), Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Luz M. SOTO-JIMENEZ (40 Austria), Robert GREENHALGH (840 United States of America), David L. PARROTT (840 United States of America), Stefan LUTZMAYER (40 Austria), Joshua G. STEFFEN (840 United States of America), Viktoria NIZHYNSKA (40 Austria), Richard MOTT (826 United Kingdom of Great Britain and Northern Ireland), Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution), Richar M. CLARK (840 United States of America) and Magnus NORDBORG (40 Austria)
Edition
GENOME BIOLOGY, LONDON, BIOMED CENTRAL LTD, 2017, 1474-760X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 13.214
RIV identification code
RIV/00216224:14740/17:00095154
Organization unit
Central European Institute of Technology
UT WoS
000400331100001
Keywords in English
Ribosomes; rRNA genes; Transcription; Epistasis; Dominance
Tags
International impact, Reviewed
Změněno: 23/3/2018 15:28, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Background: Ribosomal RNA (rRNA) accounts for the majority of the RNA in eukaryotic cells, and is encoded by hundreds to thousands of nearly identical gene copies, only a subset of which are active at any given time. In Arabidopsis thaliana, 45S rRNA genes are found in two large ribosomal DNA (rDNA) clusters and little is known about the contribution of each to the overall transcription pattern in the species. Results: By taking advantage of genome sequencing data from the 1001 Genomes Consortium, we characterize rRNA gene sequence variation within and among accessions. Notably, variation is not restricted to the pre-rRNA sequences removed during processing, but it is also present within the highly conserved ribosomal subunits. Through linkage mapping we assign these variants to a particular rDNA cluster unambiguously and use them as reporters of rDNA cluster-specific expression. We demonstrate that rDNA cluster-usage varies greatly among accessions and that rDNA cluster-specific expression and silencing is controlled via genetic interactions between entire rDNA cluster haplotypes (alleles). Conclusions: We show that rRNA gene cluster expression is controlled via complex epistatic and allelic interactions between rDNA haplotypes that apparently regulate the entire rRNA gene cluster. Furthermore, the sequence polymorphism we discovered implies that the pool of rRNA in a cell may be heterogeneous, which could have functional consequences.
Links
GBP501/12/G090, research and development project |
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