Detailed Information on Publication Record
2017
Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana
RABANAL, Fernando A., Viktoria NIZHYNSKA, Terezie MANDÁKOVÁ, Polina Yu NOVIKOVA, Martin LYSÁK et. al.Basic information
Original name
Unstable Inheritance of 45S rRNA Genes in Arabidopsis thaliana
Authors
RABANAL, Fernando A. (40 Austria), Viktoria NIZHYNSKA (40 Austria), Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Polina Yu NOVIKOVA (40 Austria), Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution), Richard MOTT (826 United Kingdom of Great Britain and Northern Ireland) and Magnus NORDBORG (40 Austria)
Edition
G3-Genes, Genomes, Genetics, USA, Bethesda, 2017, 2160-1836
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.742
RIV identification code
RIV/00216224:14740/17:00095157
Organization unit
Central European Institute of Technology
UT WoS
000398840700013
Keywords in English
ribosomes; 45S rRNA genes; natural variation; Arabidopsis thaliana
Tags
International impact, Reviewed
Změněno: 1/3/2018 14:39, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
The considerable genome size variation in Arabidopsis thaliana has been shown largely to be due to copy number variation (CNV) in 45S ribosomal RNA (rRNA) genes. Surprisingly, attempts to map this variation by means of genome-wide association studies (GWAS) failed to identify either of the two likely sources, namely the nucleolus organizer regions (NORs). Instead, GWAS implicated a trans-acting locus, as if rRNA gene CNV was a phenotype rather than a genotype. To explain these results, we investigated the inheritance and stability of rRNA gene copy number using the variety of genetic resources available in A. thaliana F2 crosses, recombinant inbred lines, the multiparent advanced-generation inter-cross population, and mutation accumulation lines. Our results clearly show that rRNA gene CNV can be mapped to the NORs themselves, with both loci contributing equally to the variation. However, NOR size is unstably inherited, and dramatic copy number changes are visible already within tens of generations, which explains why it is not possible to map the NORs using GWAS. We did not find any evidence of trans-acting loci in crosses, which is also expected since changes due to such loci would take very many generations to manifest themselves. rRNA gene copy number is thus an interesting example of missing heritabilitya trait that is heritable in pedigrees, but not in the general population.
Links
| GBP501/12/G090, research and development project |
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