Detailed Information on Publication Record
2017
3D coculture of mammary organoids with fibrospheres: A model for studying epithelial–stromal interactions during mammary branching morphogenesis
KOLEDOVÁ, ZuzanaBasic information
Original name
3D coculture of mammary organoids with fibrospheres: A model for studying epithelial–stromal interactions during mammary branching morphogenesis
Authors
KOLEDOVÁ, Zuzana (703 Slovakia, guarantor, belonging to the institution)
Edition
1st. ed. New York, 3D Cell Culture : Methods and Protocols, p. 1-11, 11 pp. Methods in Molecular Biology, volume 1612, 2017
Publisher
Humana Press
Other information
Language
English
Type of outcome
Kapitola resp. kapitoly v odborné knize
Field of Study
10601 Cell biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
printed version "print"
References:
RIV identification code
RIV/00216224:14110/17:00095165
Organization unit
Faculty of Medicine
ISBN
978-1-4939-7019-3
UT WoS
000447757200009
Keywords in English
3D culture; Branching morphogenesis; Collagen; Extracellular matrix; Fibroblasts; Mammary gland; Matrigel; Organoids; Paracrine signaling; Spheroid
Tags
Tags
International impact, Reviewed
Změněno: 27/4/2020 09:01, Mgr. Tereza Miškechová
Abstract
V originále
Mammary gland is composed of branched epithelial structure embedded within a complex stroma formed by several stromal cell types, including fibroblasts, and extracellular matrix (ECM). Development of mammary gland is tightly regulated by bidirectional epithelial–stromal interactions that include paracrine signaling, ECM remodeling and mechanosensing. Importantly, these interactions play crucial role in mammary gland homeostasis and when deregulated they contribute to tumorigenesis. Therefore, understanding the mechanisms underlying epithelial–stromal interactions is critical for elucidating regulation of normal mammary gland development and homeostasis and revealing novel strategies for breast cancer therapy. To this end, several three-dimensional (3D) cell culture models have been developed to study these interactions in vitro. In this chapter, a novel 3D organoid–fibrosphere coculture model of mammary gland is described with the capacity for studying not only the qualitative and quantitative aspects of interactions between mammary fibroblasts and epithelial organoids but also their radius and directionality
Links
EE2.3.30.0037, research and development project |
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GJ16-20031Y, research and development project |
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ROZV/20/LF/2015, interní kód MU |
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