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@inbook{1395478,
author = {Koledová, Zuzana},
address = {New York},
booktitle = {3D Cell Culture : Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-4939-7021-6_8},
edition = {1st. ed.},
editor = {Koledova Zuzana},
keywords = {3D culture; Branching morphogenesis; Collagen; Extracellular matrix; Fibroblasts; Mammary gland; Matrigel; Organoids; Paracrine signaling; Spheroid},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-4939-7019-3},
pages = {1-11},
publisher = {Humana Press},
title = {3D coculture of mammary organoids with fibrospheres: A model for studying epithelial–stromal interactions during mammary branching morphogenesis},
url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_8},
year = {2017}
}
TY - CHAP
ID - 1395478
AU - Koledová, Zuzana
PY - 2017
TI - 3D coculture of mammary organoids with fibrospheres: A model for studying epithelial–stromal interactions during mammary branching morphogenesis
VL - Methods in Molecular Biology, volume 1612
PB - Humana Press
CY - New York
SN - 9781493970193
KW - 3D culture
KW - Branching morphogenesis
KW - Collagen
KW - Extracellular matrix
KW - Fibroblasts
KW - Mammary gland
KW - Matrigel
KW - Organoids
KW - Paracrine signaling
KW - Spheroid
UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_8
L2 - https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_8
N2 - Mammary gland is composed of branched epithelial structure embedded within a complex stroma formed by several stromal cell types, including fibroblasts, and extracellular matrix (ECM). Development of mammary gland is tightly regulated by bidirectional epithelial–stromal interactions that include paracrine signaling, ECM remodeling and mechanosensing. Importantly, these interactions play crucial role in mammary gland homeostasis and when deregulated they contribute to tumorigenesis. Therefore, understanding the mechanisms underlying epithelial–stromal interactions is critical for elucidating regulation of normal mammary gland development and homeostasis and revealing novel strategies for breast cancer therapy. To this end, several three-dimensional (3D) cell culture models have been developed to study these interactions in vitro. In this chapter, a novel 3D organoid–fibrosphere coculture model of mammary gland is described with the capacity for studying not only the qualitative and quantitative aspects of interactions between mammary fibroblasts and epithelial organoids but also their radius and directionality
ER -
KOLEDOVÁ, Zuzana. 3D coculture of mammary organoids with fibrospheres: A model for studying epithelial–stromal interactions during mammary branching morphogenesis. In Koledova Zuzana. \textit{3D Cell Culture : Methods and Protocols}. 1st. ed. New York: Humana Press, 2017, p.~1-11. Methods in Molecular Biology, volume 1612. ISBN~978-1-4939-7019-3. Available from: https://dx.doi.org/10.1007/978-1-4939-7021-6\_{}8.
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