Revealing 3D Ultrastructure and Morphology of Stem Cell
Spheroids by Electron Microscopy

C 2017

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

JAROŠ, Josef, Michal PETROV, Markéta TESAŘOVÁ and Aleš HAMPL

Basic information

Original name

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Authors

JAROŠ, Josef (203 Czech Republic, guarantor, belonging to the institution), Michal PETROV (203 Czech Republic), Markéta TESAŘOVÁ (203 Czech Republic) and Aleš HAMPL (203 Czech Republic, belonging to the institution)

Edition

1st. ed. New York, 3D Cell Culture : Methods and Protocols, p. 417-431, 15 pp. Methods in Molecular Biology, volume 1612, 2017

Publisher

Humana Press

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14110/17:00095166

Organization unit

Faculty of Medicine

ISBN

978-1-4939-7019-3

DOI

http://dx.doi.org/10.1007/978-1-4939-7021-6_30

UT WoS

000447757200031

Keywords in English

3D visualization; Image reconstruction; Image segmentation; Morphology; Organoid; SBF-SEM; Scanning electron microscopy; Serial block-face; Spheroid; Stem cell; Ultrastructure

Abstract

V originále

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures

Links

EE2.3.20.0185, research and development project

Name: Centrum analýz a modelování tkání a orgánů

GA16-02702S, research and development project

Name: Materiály s nanostrukturovaným povrchem pro biomediciální aplikace

Investor: Czech Science Foundation

MUNI/M/1050/2013, interní kód MU

Name: Automatizovaná analýza elektronmikroskopických snímků pro použití v biologii a medicíně (Acronym: Analýza TEM snímků)

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

Citovat

JAROŠ, Josef, Michal PETROV, Markéta TESAŘOVÁ and Aleš HAMPL. Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy. In Zuzana Koledova. 3D Cell Culture : Methods and Protocols. 1st. ed. New York: Humana Press, 2017, p. 417-431. Methods in Molecular Biology, volume 1612. ISBN 978-1-4939-7019-3. Available from: https://dx.doi.org/10.1007/978-1-4939-7021-6_30.

@inbook{1395479,
   author = {Jaroš, Josef and Petrov, Michal and Tesařová, Markéta and Hampl, Aleš},
   address = {New York},
   booktitle = {3D Cell Culture : Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-4939-7021-6_30},
   edition = {1st. ed.},
   editor = {Zuzana Koledova},
   keywords = {3D visualization; Image reconstruction; Image segmentation; Morphology; Organoid; SBF-SEM; Scanning electron microscopy; Serial block-face; Spheroid; Stem cell; Ultrastructure},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-4939-7019-3},
   pages = {417-431},
   publisher = {Humana Press},
   title = {Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy},
   url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30},
   year = {2017}
}

TY  - CHAP
ID  - 1395479
AU  - Jaroš, Josef - Petrov, Michal - Tesařová, Markéta - Hampl, Aleš
PY  - 2017
TI  - Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy
VL  - Methods in Molecular Biology, volume 1612
PB  - Humana Press
CY  - New York
SN  - 9781493970193
KW  - 3D visualization
KW  - Image reconstruction
KW  - Image segmentation
KW  - Morphology
KW  - Organoid
KW  - SBF-SEM
KW  - Scanning electron microscopy
KW  - Serial block-face
KW  - Spheroid
KW  - Stem cell
KW  - Ultrastructure
UR  - https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30
L2  - https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30
N2  - Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures
ER  -

JAROŠ, Josef, Michal PETROV, Markéta TESAŘOVÁ and Aleš HAMPL. Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy. In Zuzana Koledova. \textit{3D Cell Culture : Methods and Protocols}. 1st. ed. New York: Humana Press, 2017, p.~417-431. Methods in Molecular Biology, volume 1612. ISBN~978-1-4939-7019-3. Available from: https://dx.doi.org/10.1007/978-1-4939-7021-6\_{}30.

Detailed Information on Publication Record