Genes of cellular components of morphogenesis in porcine
oocytes before and after IVM

J 2017

Genes of cellular components of morphogenesis in porcine oocytes before and after IVM

BUDNA, J., A. BRYJA, P. CELICHOWSKI, R. KAHAN, W. KRANC et. al.

Basic information

Original name

Genes of cellular components of morphogenesis in porcine oocytes before and after IVM

Authors

BUDNA, J. (616 Poland), A. BRYJA (616 Poland), P. CELICHOWSKI (616 Poland), R. KAHAN (616 Poland), W. KRANC (616 Poland), S. CIESIOLKA (616 Poland), M. RYBSKA (616 Poland), S. BORYS (616 Poland), Michal JEŠETA (203 Czech Republic, guarantor, belonging to the institution), D. BUKOWSKA (616 Poland), P. ANTOSIK (616 Poland), K.P. BRUSSOW (616 Poland), M. BRUSKA (616 Poland), M. NOWICKI (616 Poland), M. ZABEL (616 Poland) and B. KEMPISTY (616 Poland)

Edition

REPRODUCTION, BRISTOL, BIOSCIENTIFICA LTD, 2017, 1470-1626

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10605 Developmental biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.086

RIV identification code

RIV/00216224:14110/17:00098260

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1530/REP-17-0367

UT WoS

000412579500021

Keywords in English

oocyte

Abstract

V originále

Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating that the gene expression between immature and mature oocytes differs significantly. This study focuses on the genes responsible for the cellular components of morphogenesis within the developing oocyte. Brilliant cresyl blue (BCB) was used to determine the developmental capability of porcine oocytes. The immature oocytes (GV stage) were compared with matured oocytes (MII stage), using microarray and qRT-PCR analysis to track changes in the genetic expression profile of transcriptome genes. The data showed substantial upregulation of genes influencing oocyte's morphology, cellular migration and adhesion, intracellular communication, as well as plasticity of nervous system. Conversely, downregulation involved genes related to microtubule reorganization, regulation of adhesion, proliferation, migration and cell differentiation processes in oocytes. This suggests that most genes recruited in morphogenesis in porcine oocyte in vitro, may have cellular maturational capability, since they have a higher level of expression before the oocyte's matured form. It shows the process of oocyte maturation and developmental capacity is orchestrated by significant cellular modifications during morphogenesis.

Citovat

BUDNA, J., A. BRYJA, P. CELICHOWSKI, R. KAHAN, W. KRANC, S. CIESIOLKA, M. RYBSKA, S. BORYS, Michal JEŠETA, D. BUKOWSKA, P. ANTOSIK, K.P. BRUSSOW, M. BRUSKA, M. NOWICKI, M. ZABEL and B. KEMPISTY. Genes of cellular components of morphogenesis in porcine oocytes before and after IVM. REPRODUCTION. BRISTOL: BIOSCIENTIFICA LTD, 2017, vol. 154, No 4, p. 535-545. ISSN 1470-1626. Available from: https://dx.doi.org/10.1530/REP-17-0367.

@article{1395500,
   author = {Budna, J. and Bryja, A. and Celichowski, P. and Kahan, R. and Kranc, W. and Ciesiolka, S. and Rybska, M. and Borys, S. and Ješeta, Michal and Bukowska, D. and Antosik, P. and Brussow, K.P. and Bruska, M. and Nowicki, M. and Zabel, M. and Kempisty, B.},
   article_location = {BRISTOL},
   article_number = {4},
   doi = {http://dx.doi.org/10.1530/REP-17-0367},
   keywords = {oocyte},
   language = {eng},
   issn = {1470-1626},
   journal = {REPRODUCTION},
   title = {Genes of cellular components of morphogenesis in porcine oocytes before and after IVM},
   volume = {154},
   year = {2017}
}

TY  - JOUR
ID  - 1395500
AU  - Budna, J. - Bryja, A. - Celichowski, P. - Kahan, R. - Kranc, W. - Ciesiolka, S. - Rybska, M. - Borys, S. - Ješeta, Michal - Bukowska, D. - Antosik, P. - Brussow, K.P. - Bruska, M. - Nowicki, M. - Zabel, M. - Kempisty, B.
PY  - 2017
TI  - Genes of cellular components of morphogenesis in porcine oocytes before and after IVM
JF  - REPRODUCTION
VL  - 154
IS  - 4
SP  - 535-545
EP  - 535-545
PB  - BIOSCIENTIFICA LTD
SN  - 14701626
KW  - oocyte
N2  - Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating that the gene expression between immature and mature oocytes differs significantly. This study focuses on the genes responsible for the cellular components of morphogenesis within the developing oocyte. Brilliant cresyl blue (BCB) was used to determine the developmental capability of porcine oocytes. The immature oocytes (GV stage) were compared with matured oocytes (MII stage), using microarray and qRT-PCR analysis to track changes in the genetic expression profile of transcriptome genes. The data showed substantial upregulation of genes influencing oocyte's morphology, cellular migration and adhesion, intracellular communication, as well as plasticity of nervous system. Conversely, downregulation involved genes related to microtubule reorganization, regulation of adhesion, proliferation, migration and cell differentiation processes in oocytes. This suggests that most genes recruited in morphogenesis in porcine oocyte in vitro, may have cellular maturational capability, since they have a higher level of expression before the oocyte's matured form. It shows the process of oocyte maturation and developmental capacity is orchestrated by significant cellular modifications during morphogenesis.
ER  -

BUDNA, J., A. BRYJA, P. CELICHOWSKI, R. KAHAN, W. KRANC, S. CIESIOLKA, M. RYBSKA, S. BORYS, Michal JEŠETA, D. BUKOWSKA, P. ANTOSIK, K.P. BRUSSOW, M. BRUSKA, M. NOWICKI, M. ZABEL and B. KEMPISTY. Genes of cellular components of morphogenesis in porcine oocytes before and after IVM. \textit{REPRODUCTION}. BRISTOL: BIOSCIENTIFICA LTD, 2017, vol.~154, No~4, p.~535-545. ISSN~1470-1626. Available from: https://dx.doi.org/10.1530/REP-17-0367.

Detailed Information on Publication Record