| HORKA, M., K. SLAIS, J. SALPLACHTA and Filip RŮŽIČKA. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2017, vol. 990, OCT 16 2017, p. 185-193. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.046. |
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@article{1395637,
author = {Horka, M. and Slais, K. and Salplachta, J. and Růžička, Filip},
article_location = {Amsterdam},
article_number = {OCT 16 2017},
doi = {http://dx.doi.org/10.1016/j.aca.2017.08.046},
keywords = {Preparative isoelectric focusing; Colored microorganisms; Isoelectric points; CIEF and MALDI-TOF MS},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS},
volume = {990},
year = {2017}
}
TY - JOUR
ID - 1395637
AU - Horka, M. - Slais, K. - Salplachta, J. - Růžička, Filip
PY - 2017
TI - Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS
JF - Analytica Chimica Acta
VL - 990
IS - OCT 16 2017
SP - 185-193
EP - 185-193
PB - Elsevier Science publishers
SN - 00032670
KW - Preparative isoelectric focusing
KW - Colored microorganisms
KW - Isoelectric points
KW - CIEF and MALDI-TOF MS
N2 - Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers. Segments of the separation medium with cells were harvested by a spatula, simply purified using centrifugation and analyzed by capillary isoelectric focusing and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The determined recovery ranged from 78% to 93%. The viability of the harvested cells was verified by their cultivation.
ER -
HORKA, M., K. SLAIS, J. SALPLACHTA and Filip RŮŽIČKA. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2017, vol.~990, OCT 16 2017, p.~185-193. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.046.
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