HORKA, M., K. SLAIS, J. SALPLACHTA and Filip RŮŽIČKA. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2017, vol. 990, OCT 16 2017, p. 185-193. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.046. |
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@article{1395637, author = {Horka, M. and Slais, K. and Salplachta, J. and Růžička, Filip}, article_location = {Amsterdam}, article_number = {OCT 16 2017}, doi = {http://dx.doi.org/10.1016/j.aca.2017.08.046}, keywords = {Preparative isoelectric focusing; Colored microorganisms; Isoelectric points; CIEF and MALDI-TOF MS}, language = {eng}, issn = {0003-2670}, journal = {Analytica Chimica Acta}, title = {Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS}, volume = {990}, year = {2017} }
TY - JOUR ID - 1395637 AU - Horka, M. - Slais, K. - Salplachta, J. - Růžička, Filip PY - 2017 TI - Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS JF - Analytica Chimica Acta VL - 990 IS - OCT 16 2017 SP - 185-193 EP - 185-193 PB - Elsevier Science publishers SN - 00032670 KW - Preparative isoelectric focusing KW - Colored microorganisms KW - Isoelectric points KW - CIEF and MALDI-TOF MS N2 - Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers. Segments of the separation medium with cells were harvested by a spatula, simply purified using centrifugation and analyzed by capillary isoelectric focusing and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The determined recovery ranged from 78% to 93%. The viability of the harvested cells was verified by their cultivation. ER -
HORKA, M., K. SLAIS, J. SALPLACHTA and Filip RŮŽIČKA. Preparative isoelectric focusing of microorganisms in cellulose-based separation medium and subsequent analysis by CIEF and MALDI-TOF MS. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2017, vol.~990, OCT 16 2017, p.~185-193. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.046.
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