Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

REBROŠOVÁ, Katarína, M. SILER, O. SAMEK, Filip RŮŽIČKA, S. BERNATOVA, J. JEZEK, P. ZEMANEK and Veronika HOLÁ. Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy. FUTURE MICROBIOLOGY. LONDON: FUTURE MEDICINE LTD, 2017, vol. 12, No 10, p. 881-890. ISSN 1746-0913. Available from: https://dx.doi.org/10.2217/fmb-2016-0224.

Basic information

• Original name: Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy
• Authors: REBROŠOVÁ, Katarína (703 Slovakia, belonging to the institution), M. SILER (203 Czech Republic), O. SAMEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, guarantor, belonging to the institution), S. BERNATOVA (203 Czech Republic), J. JEZEK (203 Czech Republic), P. ZEMANEK (203 Czech Republic) and Veronika HOLÁ (203 Czech Republic, belonging to the institution).
• Edition: FUTURE MICROBIOLOGY, LONDON, FUTURE MEDICINE LTD, 2017, 1746-0913.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10606 Microbiology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.190
• RIV identification code: RIV/00216224:14110/17:00095673
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.2217/fmb-2016-0224
• UT WoS: 000408437400008
• Keywords in English: Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis; Staphyococcus aureus
• Tags: EL OK
• Tags: International impact, Reviewed

Abstract

Aim: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.

Links

• NV16-29916A, research and development project: Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu
• NV16-31593A, research and development project: Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí