Rapid identification of staphylococci by Raman spectroscopy

J 2017

Rapid identification of staphylococci by Raman spectroscopy

REBROŠOVÁ, Katarína, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, S. BERNATOVÁ et. al.

Basic information

Original name

Rapid identification of staphylococci by Raman spectroscopy

Authors

REBROŠOVÁ, Katarína (703 Slovakia, belonging to the institution), M. ŠILER (203 Czech Republic), O. SAMEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), S. BERNATOVÁ (203 Czech Republic), Veronika HOLÁ (203 Czech Republic, belonging to the institution), J. JEŽEK (203 Czech Republic), P. ZEMÁNEK (203 Czech Republic), J. SOKOLOVÁ (203 Czech Republic) and P. PETRÁŠ (203 Czech Republic)

Edition

Scientific Reports, London, Nature Publishing Group, 2017, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.122

RIV identification code

RIV/00216224:14110/17:00095674

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-017-13940-w

UT WoS

000414233700066

Keywords in English

bacterium colony; human major clinical study; nonhuman ; Raman spectrometry; Staphylococcus

Abstract

V originále

Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.

Links

MUNI/A/0955/2016, interní kód MU

Name: Optimalizace diagnostiky původců nozokomiálních nákaz testování nových možností antimikrobiální terapie

Investor: Masaryk University, Category A

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

NV16-31593A, research and development project

Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

REBROŠOVÁ, Katarína, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, S. BERNATOVÁ, Veronika HOLÁ, J. JEŽEK, P. ZEMÁNEK, J. SOKOLOVÁ and P. PETRÁŠ. Rapid identification of staphylococci by Raman spectroscopy. Scientific Reports. London: Nature Publishing Group, 2017, vol. 7, No 1, p. 1-8. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-017-13940-w.

@article{1395649,
   author = {Rebrošová, Katarína and Šiler, M. and Samek, O. and Růžička, Filip and Bernatová, S. and Holá, Veronika and Ježek, J. and Zemánek, P. and Sokolová, J. and Petráš, P.},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-017-13940-w},
   keywords = {bacterium colony; human major clinical study; nonhuman ; Raman spectrometry; Staphylococcus},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Rapid identification of staphylococci by Raman spectroscopy},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1395649
AU  - Rebrošová, Katarína - Šiler, M. - Samek, O. - Růžička, Filip - Bernatová, S. - Holá, Veronika - Ježek, J. - Zemánek, P. - Sokolová, J. - Petráš, P.
PY  - 2017
TI  - Rapid identification of staphylococci by Raman spectroscopy
JF  - Scientific Reports
VL  - 7
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Nature Publishing Group
SN  - 20452322
KW  - bacterium colony
KW  - human major clinical study
KW  - nonhuman
KW  - Raman spectrometry
KW  - Staphylococcus
N2  - Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
ER  -

REBROŠOVÁ, Katarína, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, S. BERNATOVÁ, Veronika HOLÁ, J. JEŽEK, P. ZEMÁNEK, J. SOKOLOVÁ and P. PETRÁŠ. Rapid identification of staphylococci by Raman spectroscopy. \textit{Scientific Reports}. London: Nature Publishing Group, 2017, vol.~7, No~1, p.~1-8. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-017-13940-w.

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