| REBROŠOVÁ, Katarína, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, S. BERNATOVÁ, Veronika HOLÁ, J. JEŽEK, P. ZEMÁNEK, J. SOKOLOVÁ and P. PETRÁŠ. Rapid identification of staphylococci by Raman spectroscopy. Scientific Reports. London: Nature Publishing Group, 2017, vol. 7, No 1, p. 1-8. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-017-13940-w. |
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@article{1395649,
author = {Rebrošová, Katarína and Šiler, M. and Samek, O. and Růžička, Filip and Bernatová, S. and Holá, Veronika and Ježek, J. and Zemánek, P. and Sokolová, J. and Petráš, P.},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-017-13940-w},
keywords = {bacterium colony; human major clinical study; nonhuman ; Raman spectrometry; Staphylococcus},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Rapid identification of staphylococci by Raman spectroscopy},
volume = {7},
year = {2017}
}
TY - JOUR
ID - 1395649
AU - Rebrošová, Katarína - Šiler, M. - Samek, O. - Růžička, Filip - Bernatová, S. - Holá, Veronika - Ježek, J. - Zemánek, P. - Sokolová, J. - Petráš, P.
PY - 2017
TI - Rapid identification of staphylococci by Raman spectroscopy
JF - Scientific Reports
VL - 7
IS - 1
SP - 1-8
EP - 1-8
PB - Nature Publishing Group
SN - 20452322
KW - bacterium colony
KW - human major clinical study
KW - nonhuman
KW - Raman spectrometry
KW - Staphylococcus
N2 - Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
ER -
REBROŠOVÁ, Katarína, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, S. BERNATOVÁ, Veronika HOLÁ, J. JEŽEK, P. ZEMÁNEK, J. SOKOLOVÁ and P. PETRÁŠ. Rapid identification of staphylococci by Raman spectroscopy. \textit{Scientific Reports}. London: Nature Publishing Group, 2017, vol.~7, No~1, p.~1-8. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-017-13940-w.
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