Myeloperoxidase (MPO) gene variability in patients with
recurrent aphthous stomatitis

MASOPUSTOVÁ, Lucie, Petra BOŘILOVÁ LINHARTOVÁ, Simona SLEZÁKOVÁ, Jiřina BÁRTOVÁ, Jitka PETANOVÁ, Pavel KUKLÍNEK, Antonín FASSMANN and Lydie IZAKOVIČOVÁ HOLLÁ. Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis. In XVIII. Setkání biochemiků a molekulárních biologů, Brno. 2017. ISBN 978-80-210-8765-1.

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TY  - CONF
ID  - 1395667
AU  - Masopustová, Lucie - Bořilová Linhartová, Petra - Slezáková, Simona - Bártová, Jiřina - Petanová, Jitka - Kuklínek, Pavel - Fassmann, Antonín - Izakovičová Hollá, Lydie
PY  - 2017
TI  - Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis
SN  - 9788021087651
KW  - myeloperoxidase
KW  - association studies
KW  - candidate gene
KW  - genetic
KW  - immunity
KW  - oral ulcer
KW  - polymorphisms
N2  - Introduction: Recurrent aphthous stomatitis (RAS) is a multifactorial disease, the exact etiological factor(s) are not understood. One of the contributing factors is oxidative stress caused by an imbalance between reactive oxygen species (ROS) and an antioxidant system. Myeloperoxidase (MPO) is an enzyme utilizing the oxidation potential of superoxides and hydrogen peroxide to convert chloride ions to chlorine and another ROS. The aim of this study was to determine polymorphisms in MPO (rs2243828, rs2759, rs7208693, rs2333227) in RAS patients and control subjects in Czech population. Materials and methods: We performed a case–control study in 275 subjects (70 patients with recurrent aphthous stomatitis and 205 healthy individuals). Polymorphisms were detected by using real-time PCR with the use of TaqMan probe and RFLP-PCR with restriction analysis. Results: No statistically significant differences were found in allelic and genotypic frequencies of MPO polymorphisms (rs2243828, rs2759, rs7208693, rs2333227) among patients with RAS and controls. However statistical significance was found in SNP MPO (rs2333227, rs2243828) when comparing the duration of lesions, difference is between individuals with the GG genotype compared to carriers of AA + AG genotypes (P = 0.02). No significant association was detected between MPO haplotypes and RAS. Conclusion: When comparing our results with studies in the Turkish population, we concluded that variability in the myeloperoxidase gene may not play a role in the development of the disease. Acknowledgements: This study was supported by grant AZV 15-29336A, GAČR GB14-37368G and project MUNI / A / 0948/2016.
ER  -

Basic information
Original name	Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis
Authors	MASOPUSTOVÁ, Lucie (203 Czech Republic, belonging to the institution), Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, belonging to the institution), Simona SLEZÁKOVÁ (203 Czech Republic, belonging to the institution), Jiřina BÁRTOVÁ (203 Czech Republic), Jitka PETANOVÁ (203 Czech Republic), Pavel KUKLÍNEK (203 Czech Republic, belonging to the institution), Antonín FASSMANN (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition	XVIII. Setkání biochemiků a molekulárních biologů, Brno, 2017.

Other information
Original language	English
Type of outcome	Conference abstract
Field of Study	30208 Dentistry, oral surgery and medicine
Country of publisher	Czech Republic
Confidentiality degree	is not subject to a state or trade secret
RIV identification code	RIV/00216224:14110/17:00100749
Organization unit	Faculty of Medicine
ISBN	978-80-210-8765-1
Keywords in English	myeloperoxidase; association studies; candidate gene; genetic; immunity; oral ulcer; polymorphisms
Changed by	Changed by: Mgr. et Mgr. Simona Slezáková, Ph.D., učo 357612. Changed: 18/1/2019 14:06.

Abstract

Introduction: Recurrent aphthous stomatitis (RAS) is a multifactorial disease, the exact etiological factor(s) are not understood. One of the contributing factors is oxidative stress caused by an imbalance between reactive oxygen species (ROS) and an antioxidant system. Myeloperoxidase (MPO) is an enzyme utilizing the oxidation potential of superoxides and hydrogen peroxide to convert chloride ions to chlorine and another ROS. The aim of this study was to determine polymorphisms in MPO (rs2243828, rs2759, rs7208693, rs2333227) in RAS patients and control subjects in Czech population. Materials and methods: We performed a case–control study in 275 subjects (70 patients with recurrent aphthous stomatitis and 205 healthy individuals). Polymorphisms were detected by using real-time PCR with the use of TaqMan probe and RFLP-PCR with restriction analysis. Results: No statistically significant differences were found in allelic and genotypic frequencies of MPO polymorphisms (rs2243828, rs2759, rs7208693, rs2333227) among patients with RAS and controls. However statistical significance was found in SNP MPO (rs2333227, rs2243828) when comparing the duration of lesions, difference is between individuals with the GG genotype compared to carriers of AA + AG genotypes (P = 0.02). No significant association was detected between MPO haplotypes and RAS. Conclusion: When comparing our results with studies in the Turkish population, we concluded that variability in the myeloperoxidase gene may not play a role in the development of the disease. Acknowledgements: This study was supported by grant AZV 15-29336A, GAČR GB14-37368G and project MUNI / A / 0948/2016.

Links
GB14-37368G, research and development project	Name: Centrum orofaciálního vývoje a regenerace
GB14-37368G, research and development project	Investor: Czech Science Foundation
MUNI/A/0948/2016, interní kód MU	Name: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba
MUNI/A/0948/2016, interní kód MU	Investor: Masaryk University, Category A

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Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis

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