a 2017

Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis

JÁNOŠ, Július, Petra BOŘILOVÁ LINHARTOVÁ, Hana POSKEROVÁ and Lydie IZAKOVIČOVÁ HOLLÁ

Basic information

Original name

Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis

Authors

Edition

XVIII. Setkání biochemiků a molekulárních biologů, Brno, 2017

Other information

Type of outcome

Konferenční abstrakt

Confidentiality degree

není předmětem státního či obchodního tajemství

ISBN

978-80-210-8765-1

Keywords (in Czech)

purinergní receptor; diabetes mellitus; parodontitida; chronický zápal; genová variabilita; polymorfismus

Keywords in English

purinergic receptor; diabetes mellitus; periodontitis; chronic inflammation; gene variability; polymorphism

Abstract

V originále

Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P<0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation. Acknowledgements: The study was supported by funds provided by the Faculty of Medicine MU to junior researcher Petra Borilova Linhartova, grant GACR GB14-37368G and project MUNI/A/0948/2016.

Links

GB14-37368G, research and development project
Name: Centrum orofaciálního vývoje a regenerace
Investor: Czech Science Foundation
MUNI/A/0948/2016, interní kód MU
Name: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba
Investor: Masaryk University, Category A
ROZV/25/LF/2017, interní kód MU
Name: LF - Příspěvek na IP 2017
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects