Detailed Information on Publication Record
2017
The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity
HOŠEK, Jan, Kristýna ŠEBRLOVÁ, Petra KAUCKÁ, Ondřej PEŠ, Eva TÁBORSKÁ et. al.Basic information
Original name
The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity
Authors
HOŠEK, Jan (203 Czech Republic), Kristýna ŠEBRLOVÁ (203 Czech Republic, belonging to the institution), Petra KAUCKÁ (203 Czech Republic), Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution) and Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution)
Edition
Acta Veterinaria Brno, Brno, Veterinární a farmaceutická univerzita Brno, 2017, 0001-7213
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
40300 4.3 Veterinary science
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.422
RIV identification code
RIV/00216224:14110/17:00098458
Organization unit
Faculty of Medicine
UT WoS
000416306200004
Keywords in English
Cyclooxygenase; cytotoxicity; inflammation; TNF-alpha.
Tags
Tags
International impact, Reviewed
Změněno: 20/3/2018 18:44, Soňa Böhmová
Abstract
V originále
Quaternary benzophenanthridine alkaloids are known to have a wide range of biological effects, including antimicrobial, antifungal, anti-inflammatory, and antitumour activities. However, only sanguinarine and chelerythrine have been studied intensively. The aim of this study was to evaluate the anti-inflammatory potential of the five minor quaternary benzophenanthridine alkaloids sanguilutine, sanguirubine, chelirubine, chelilutine, and macarpine in vitro and to compare them with more thoroughly studied sanguinarine and chelerythrine. Before making cell-based assays, the cytotoxicity of the alkaloids was evaluated. The anti-inflammatory potential of the chosen alkaloids was evaluated as for their ability to modulate the lipopolysaccharide-induced secretion of tumour necrosis factor alpha (TNF-alpha) in the macrophage-like cell line THP-1. The cyclooxygenase (COX)-1 and COX-2 inhibitory activities were also measured. The results indicate that the presence of a methylenedioxy ring attached at carbon (C)7-C8 is important for reducing the secretion of TNF-alpha. Interestingly, this effect did not show a simple dependence on concentration. The selected alkaloids showed little or no anti-COX activity. The results obtained from the present experiments may provide additional information useful in understanding the structure-to-activity relationship of the quaternary benzophenanthridine alkaloids. The anti-inflammatory potential and the cytotoxic effect are driven by the presence of a methylenedioxy ring attached at C7-C8 and C2-C3, respectively.
Links
| MUNI/A/1205/2016, interní kód MU |
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