MEADER, Ellie, Tomáš BÁRTA, Dario MELGUIZO-SANCHIS, Katarzyna TILGNER, David MONTANER, Ashraf A. EL-HAROUNI, Lyle ARMSTRONG and Majlinda LAKO. Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs. Online. Stem Cells. Hoboken: Wiley-Blackwell, 2018, vol. 36, No 1, p. 55-64. ISSN 1066-5099. Available from: https://dx.doi.org/10.1002/stem.2724. [citováno 2024-04-23]
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Basic information
Original name Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs
Authors MEADER, Ellie (826 United Kingdom of Great Britain and Northern Ireland), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Dario MELGUIZO-SANCHIS (826 United Kingdom of Great Britain and Northern Ireland), Katarzyna TILGNER (826 United Kingdom of Great Britain and Northern Ireland), David MONTANER (724 Spain), Ashraf A. EL-HAROUNI (682 Saudi Arabia), Lyle ARMSTRONG (826 United Kingdom of Great Britain and Northern Ireland) and Majlinda LAKO (826 United Kingdom of Great Britain and Northern Ireland, guarantor)
Edition Stem Cells, Hoboken, Wiley-Blackwell, 2018, 1066-5099.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.614
RIV identification code RIV/00216224:14110/18:00102112
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1002/stem.2724
UT WoS 000418942500007
Keywords in English Human embryonic stem cells; miRNAs; Epithelial-mesenchymal transition; Hematopoietic differentiation
Tags 14110517, EL OK, rivok
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 10/2/2019 17:25.
Abstract
Hematopoietic stem cells derived from pluripotent stem cells could be used as an alternative to bone marrow transplants. Deriving these has been a long-term goal for researchers. However, the success of these efforts has been limited with the cells produced able to engraft in the bone marrow of recipient animals only in very low numbers. There is evidence that defects in the migratory and homing capacity of the cells are due to mis-regulation of miRNA expression and are responsible for their failure to engraft. We compared the miRNA expression profile of hematopoietic progenitors derived from pluripotent stem cells to those derived from bone marrow and found that numerous miRNAs are too highly expressed in hematopoietic progenitors derived from pluripotent stem cells, and that most of these are inhibitors of epithelial-mesenchymal transition or metastasis (including miR-200b, miR-200c, miR-205, miR-148a, and miR-424). We hypothesize that the high expression of these factors, which promote an adherent phenotype, may be causing the defect in hematopoietic differentiation. However, inhibiting these miRNAs, individually or in multiplex, was insufficient to improve hematopoietic differentiation in vitro, suggesting that other miRNAs and/or genes may be involved in this process.
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