J 2018

Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs

MEADER, Ellie, Tomáš BÁRTA, Dario MELGUIZO-SANCHIS, Katarzyna TILGNER, David MONTANER et. al.

Basic information

Original name

Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs

Authors

MEADER, Ellie (826 United Kingdom of Great Britain and Northern Ireland), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Dario MELGUIZO-SANCHIS (826 United Kingdom of Great Britain and Northern Ireland), Katarzyna TILGNER (826 United Kingdom of Great Britain and Northern Ireland), David MONTANER (724 Spain), Ashraf A. EL-HAROUNI (682 Saudi Arabia), Lyle ARMSTRONG (826 United Kingdom of Great Britain and Northern Ireland) and Majlinda LAKO (826 United Kingdom of Great Britain and Northern Ireland, guarantor)

Edition

Stem Cells, Hoboken, Wiley-Blackwell, 2018, 1066-5099

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.614

RIV identification code

RIV/00216224:14110/18:00102112

Organization unit

Faculty of Medicine

DOI

UT WoS

000418942500007

Keywords in English

Human embryonic stem cells; miRNAs; Epithelial-mesenchymal transition; Hematopoietic differentiation

Tags

Tags

International impact, Reviewed
Změněno: 10/2/2019 17:25, Soňa Böhmová

Abstract

V originále

Hematopoietic stem cells derived from pluripotent stem cells could be used as an alternative to bone marrow transplants. Deriving these has been a long-term goal for researchers. However, the success of these efforts has been limited with the cells produced able to engraft in the bone marrow of recipient animals only in very low numbers. There is evidence that defects in the migratory and homing capacity of the cells are due to mis-regulation of miRNA expression and are responsible for their failure to engraft. We compared the miRNA expression profile of hematopoietic progenitors derived from pluripotent stem cells to those derived from bone marrow and found that numerous miRNAs are too highly expressed in hematopoietic progenitors derived from pluripotent stem cells, and that most of these are inhibitors of epithelial-mesenchymal transition or metastasis (including miR-200b, miR-200c, miR-205, miR-148a, and miR-424). We hypothesize that the high expression of these factors, which promote an adherent phenotype, may be causing the defect in hematopoietic differentiation. However, inhibiting these miRNAs, individually or in multiplex, was insufficient to improve hematopoietic differentiation in vitro, suggesting that other miRNAs and/or genes may be involved in this process.