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@article{1396967,
author = {Králíková, Michaela and Crha, Igor and Huser, Martin and Melounová, Jitka and Žáková, Jana and Matejovičová, Milena and Ventruba, Pavel},
article_location = {HOBOKEN},
article_number = {7},
doi = {http://dx.doi.org/10.1111/and.12702},
keywords = {glutathione; homocysteine; seminal plasma; spermatozoa; thiols},
language = {eng},
issn = {0303-4569},
journal = {ANDROLOGIA},
title = {The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis},
volume = {49},
year = {2017}
}
TY - JOUR
ID - 1396967
AU - Králíková, Michaela - Crha, Igor - Huser, Martin - Melounová, Jitka - Žáková, Jana - Matejovičová, Milena - Ventruba, Pavel
PY - 2017
TI - The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis
JF - ANDROLOGIA
VL - 49
IS - 7
SP - 1-5
EP - 1-5
PB - WILEY
SN - 03034569
KW - glutathione
KW - homocysteine
KW - seminal plasma
KW - spermatozoa
KW - thiols
N2 - The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.
ER -
KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ, Milena MATEJOVIČOVÁ and Pavel VENTRUBA. The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis. \textit{ANDROLOGIA}. HOBOKEN: WILEY, 2017, vol.~49, No~7, p.~1-5. ISSN~0303-4569. Available from: https://dx.doi.org/10.1111/and.12702.
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