The intracellular concentration of homocysteine and related
thiols is negatively correlated to sperm quality after highly
effective method of sperm lysis

J 2017

The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis

KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ et. al.

Basic information

Original name

The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis

Authors

KRÁLÍKOVÁ, Michaela (203 Czech Republic, guarantor, belonging to the institution), Igor CRHA (203 Czech Republic, belonging to the institution), Martin HUSER (203 Czech Republic, belonging to the institution), Jitka MELOUNOVÁ (203 Czech Republic, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Milena MATEJOVIČOVÁ (203 Czech Republic, belonging to the institution) and Pavel VENTRUBA (203 Czech Republic, belonging to the institution)

Edition

ANDROLOGIA, HOBOKEN, WILEY, 2017, 0303-4569

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30202 Endocrinology and metabolism

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.588

RIV identification code

RIV/00216224:14110/17:00095676

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/and.12702

UT WoS

000408626900011

Keywords in English

glutathione; homocysteine; seminal plasma; spermatozoa; thiols

Abstract

V originále

The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.

Links

NS9661, research and development project

Name: Vliv metabolismu homocysteinu na poruchy plodnosti.

Investor: Ministry of Health of the CR

Citovat

KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ, Milena MATEJOVIČOVÁ and Pavel VENTRUBA. The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis. ANDROLOGIA. HOBOKEN: WILEY, 2017, vol. 49, No 7, p. 1-5. ISSN 0303-4569. Available from: https://dx.doi.org/10.1111/and.12702.

@article{1396967,
   author = {Králíková, Michaela and Crha, Igor and Huser, Martin and Melounová, Jitka and Žáková, Jana and Matejovičová, Milena and Ventruba, Pavel},
   article_location = {HOBOKEN},
   article_number = {7},
   doi = {http://dx.doi.org/10.1111/and.12702},
   keywords = {glutathione; homocysteine; seminal plasma; spermatozoa; thiols},
   language = {eng},
   issn = {0303-4569},
   journal = {ANDROLOGIA},
   title = {The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis},
   volume = {49},
   year = {2017}
}

TY  - JOUR
ID  - 1396967
AU  - Králíková, Michaela - Crha, Igor - Huser, Martin - Melounová, Jitka - Žáková, Jana - Matejovičová, Milena - Ventruba, Pavel
PY  - 2017
TI  - The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis
JF  - ANDROLOGIA
VL  - 49
IS  - 7
SP  - 1-5
EP  - 1-5
PB  - WILEY
SN  - 03034569
KW  - glutathione
KW  - homocysteine
KW  - seminal plasma
KW  - spermatozoa
KW  - thiols
N2  - The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.
ER  -

KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ, Milena MATEJOVIČOVÁ and Pavel VENTRUBA. The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis. \textit{ANDROLOGIA}. HOBOKEN: WILEY, 2017, vol.~49, No~7, p.~1-5. ISSN~0303-4569. Available from: https://dx.doi.org/10.1111/and.12702.

Detailed Information on Publication Record