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@article{1397142,
author = {Sisáková, Alexandra and Altmannová, Veronika and Šebesta, Marek and Krejčí, Lumír},
article_location = {LONDON},
article_number = {90},
doi = {http://dx.doi.org/10.1186/s12915-017-0429-8},
keywords = {Replication factor C; Proliferating cell nuclear antigen; Mus81 complex; Replication; Recombination},
language = {eng},
issn = {1741-7007},
journal = {BMC BIOLOGY},
title = {Role of PCNA and RFC in promoting Mus81-complex activity},
volume = {15},
year = {2017}
}
TY - JOUR
ID - 1397142
AU - Sisáková, Alexandra - Altmannová, Veronika - Šebesta, Marek - Krejčí, Lumír
PY - 2017
TI - Role of PCNA and RFC in promoting Mus81-complex activity
JF - BMC BIOLOGY
VL - 15
IS - 90
SP - 1-17
EP - 1-17
PB - BIOMED CENTRAL LTD
SN - 17417007
KW - Replication factor C
KW - Proliferating cell nuclear antigen
KW - Mus81 complex
KW - Replication
KW - Recombination
N2 - Background: Proper DNA replication is essential for faithful transmission of the genome. However, replication stress has serious impact on the integrity of the cell, leading to stalling or collapse of replication forks, and has been determined as a driving force of carcinogenesis. Mus81-Mms4 complex is a structure-specific endonuclease previously shown to be involved in processing of aberrant replication intermediates and promotes POLD3-dependent DNA synthesis via break-induced replication. However, how replication components might be involved in this process is not known. Results: Herein, we show the interaction and robust stimulation of Mus81-Mms4 nuclease activity by heteropentameric replication factor C(RFC) complex, the processivity factor of replicative DNA polymerases that is responsible for loading of proliferating cell nuclear antigen (PCNA) during DNA replication and repair. This stimulation is enhanced by RFC-dependent ATP hydrolysis and by PCNA loading on the DNA. Moreover, this stimulation is not specific to Rfc1, the largest of subunit of this complex, thus indicating that alternative clamp loaders may also play a role in the stimulation. We also observed a targeting of Mus81 by RFC to the nick-containing DNA substrate and we provide further evidence that indicates cooperation between Mus81 and the RFC complex in the repair of DNA lesions generated by various DNA-damaging agents. Conclusions: Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
ER -
SISÁKOVÁ, Alexandra, Veronika ALTMANNOVÁ, Marek ŠEBESTA and Lumír KREJČÍ. Role of PCNA and RFC in promoting Mus81-complex activity. \textit{BMC BIOLOGY}. LONDON: BIOMED CENTRAL LTD, 2017, vol.~15, No~90, p.~1-17. ISSN~1741-7007. Available from: https://dx.doi.org/10.1186/s12915-017-0429-8.
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