Detailed Information on Publication Record
2017
New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection
ĎURČ, Pavol, František FORET, Eva POKOJOVÁ, Lukáš HOMOLA, Jana SKŘIČKOVÁ et. al.Basic information
Original name
New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection
Authors
ĎURČ, Pavol (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution), Eva POKOJOVÁ (203 Czech Republic), Lukáš HOMOLA (203 Czech Republic), Jana SKŘIČKOVÁ (203 Czech Republic, guarantor), Vladimír HEROUT (203 Czech Republic), Milan DASTYCH (203 Czech Republic), Hana VINOHRADSKÁ (203 Czech Republic) and Petr KUBÁŇ (203 Czech Republic, belonging to the institution)
Edition
Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2017, 1618-2642
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.307
RIV identification code
RIV/00216224:14110/17:00095220
Organization unit
Faculty of Medicine
UT WoS
000401408300002
Keywords in English
Cystic fibrosis; Capillary electrophoresis; Sweat; Skin wipe; Ion ratio; Diagnosis
Tags
International impact, Reviewed
Změněno: 20/3/2018 16:46, Soňa Böhmová
Abstract
V originále
A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless conductometric detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 mu L deionized water. The skin-wipe sample is then extracted for 3 min into 400 mu L deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless conductometric detection using a double opposite end injection. A 20 mmol/L L-histidine/2-(N-'morpholino) ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants-ten adult patients with CF (25-50 years old), ten pediatric patients with CF (1-15 years old), and ten healthy control individuals (1-18 years old)-were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient's age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
Links
| GA13-21919S, research and development project |
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| LQ1601, research and development project |
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