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@article{1397246, author = {Ďurč, Pavol and Foret, František and Pokojová, Eva and Homola, Lukáš and Skřičková, Jana and Herout, Vladimír and Dastych, Milan and Vinohradská, Hana and Kubáň, Petr}, article_location = {HEIDELBERG}, article_number = {14}, doi = {http://dx.doi.org/10.1007/s00216-017-0318-6}, keywords = {Cystic fibrosis; Capillary electrophoresis; Sweat; Skin wipe; Ion ratio; Diagnosis}, language = {eng}, issn = {1618-2642}, journal = {Analytical and Bioanalytical chemistry}, title = {New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection}, volume = {409}, year = {2017} }
TY - JOUR ID - 1397246 AU - Ďurč, Pavol - Foret, František - Pokojová, Eva - Homola, Lukáš - Skřičková, Jana - Herout, Vladimír - Dastych, Milan - Vinohradská, Hana - Kubáň, Petr PY - 2017 TI - New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection JF - Analytical and Bioanalytical chemistry VL - 409 IS - 14 SP - 3507-3514 EP - 3507-3514 PB - SPRINGER HEIDELBERG SN - 16182642 KW - Cystic fibrosis KW - Capillary electrophoresis KW - Sweat KW - Skin wipe KW - Ion ratio KW - Diagnosis N2 - A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless conductometric detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 mu L deionized water. The skin-wipe sample is then extracted for 3 min into 400 mu L deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless conductometric detection using a double opposite end injection. A 20 mmol/L L-histidine/2-(N-'morpholino) ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants-ten adult patients with CF (25-50 years old), ten pediatric patients with CF (1-15 years old), and ten healthy control individuals (1-18 years old)-were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient's age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test. ER -
ĎURČ, Pavol, František FORET, Eva POKOJOVÁ, Lukáš HOMOLA, Jana SKŘIČKOVÁ, Vladimír HEROUT, Milan DASTYCH, Hana VINOHRADSKÁ and Petr KUBÁŇ. New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2017, vol.~409, No~14, p.~3507-3514. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-017-0318-6.
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