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@article{1397246,
author = {Ďurč, Pavol and Foret, František and Pokojová, Eva and Homola, Lukáš and Skřičková, Jana and Herout, Vladimír and Dastych, Milan and Vinohradská, Hana and Kubáň, Petr},
article_location = {HEIDELBERG},
article_number = {14},
doi = {http://dx.doi.org/10.1007/s00216-017-0318-6},
keywords = {Cystic fibrosis; Capillary electrophoresis; Sweat; Skin wipe; Ion ratio; Diagnosis},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical chemistry},
title = {New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection},
volume = {409},
year = {2017}
}
TY - JOUR
ID - 1397246
AU - Ďurč, Pavol - Foret, František - Pokojová, Eva - Homola, Lukáš - Skřičková, Jana - Herout, Vladimír - Dastych, Milan - Vinohradská, Hana - Kubáň, Petr
PY - 2017
TI - New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection
JF - Analytical and Bioanalytical chemistry
VL - 409
IS - 14
SP - 3507-3514
EP - 3507-3514
PB - SPRINGER HEIDELBERG
SN - 16182642
KW - Cystic fibrosis
KW - Capillary electrophoresis
KW - Sweat
KW - Skin wipe
KW - Ion ratio
KW - Diagnosis
N2 - A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless conductometric detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 mu L deionized water. The skin-wipe sample is then extracted for 3 min into 400 mu L deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless conductometric detection using a double opposite end injection. A 20 mmol/L L-histidine/2-(N-'morpholino) ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants-ten adult patients with CF (25-50 years old), ten pediatric patients with CF (1-15 years old), and ten healthy control individuals (1-18 years old)-were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient's age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.
ER -
ĎURČ, Pavol, František FORET, Eva POKOJOVÁ, Lukáš HOMOLA, Jana SKŘIČKOVÁ, Vladimír HEROUT, Milan DASTYCH, Hana VINOHRADSKÁ and Petr KUBÁŇ. New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, vol.~409, No~14, p.~3507-3514. ISSN~1618-2642. doi:10.1007/s00216-017-0318-6. 2017.
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