GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA and Antonín HLAVÁČEK. Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools. In 2017 MRS Fall Meeting & Exhibit. 2017.
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Basic information
Original name Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools
Authors GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA and Antonín HLAVÁČEK.
Edition 2017 MRS Fall Meeting & Exhibit, 2017.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization unit Central European Institute of Technology
Keywords in English photon-upconversion nanoparticle; electrophoretic separation; single-molecule analysis; upconversion-linked immunosorbent assay
Tags International impact
Changed by Changed by: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Changed: 24/2/2019 17:33.
Abstract
Conventional fluorescence spectroscopy is limited by autofluorescence and light scattering of the surrounding matrix. This optical background interference can be elegantly avoided by using photon-upconversion nanoparticles (UCNPs) that emit short-wavelength light under near-infrared (NIR, 980 nm) excitation (anti-Stokes emission). I will describe new nano-analytical techniques taking full advantage of UCNPs. Agarose gel electrophoresis enables the separation and purification of UCNPs that can then be detected in the gel by a 980-nm laser scanner. The purifiation of UCNPs bioconjugates has the potential to improve many bioanalytical applications. Such well-defined UCNPs can be used to replace conventional enzyme-mediated amplification systems in microtiter plate immunoassays. For example, an upconversion-linked immunoassay (ULISA) allowed for the sensitive detection of the pharmaceutical diclofenac in environmental water samples. UCNPs can be detected at the single nanoparticle level using a relatively simple wide-field upconversion microscope. In this way, it is possible to implement a single molecule sandwich immunoassay for the detection of diagnostic markers such as prostate-specific antigen (PSA).
Links
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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