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@proceedings{1398200, author = {Gorris, HansandHeiner and Mickert, Matthias Jürgen and Farka, Zdeněk and Hlaváček, Antonín}, booktitle = {2017 MRS Fall Meeting & Exhibit}, keywords = {photon-upconversion nanoparticle; electrophoretic separation; single-molecule analysis; upconversion-linked immunosorbent assay}, language = {eng}, title = {Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools}, url = {http://www.mrs.org/fall2017-symposium-sessions?code=NM07}, year = {2017} }
TY - CONF ID - 1398200 AU - Gorris, Hans-Heiner - Mickert, Matthias Jürgen - Farka, Zdeněk - Hlaváček, Antonín PY - 2017 TI - Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools KW - photon-upconversion nanoparticle KW - electrophoretic separation KW - single-molecule analysis KW - upconversion-linked immunosorbent assay UR - http://www.mrs.org/fall2017-symposium-sessions?code=NM07 N2 - Conventional fluorescence spectroscopy is limited by autofluorescence and light scattering of the surrounding matrix. This optical background interference can be elegantly avoided by using photon-upconversion nanoparticles (UCNPs) that emit short-wavelength light under near-infrared (NIR, 980 nm) excitation (anti-Stokes emission). I will describe new nano-analytical techniques taking full advantage of UCNPs. Agarose gel electrophoresis enables the separation and purification of UCNPs that can then be detected in the gel by a 980-nm laser scanner. The purifiation of UCNPs bioconjugates has the potential to improve many bioanalytical applications. Such well-defined UCNPs can be used to replace conventional enzyme-mediated amplification systems in microtiter plate immunoassays. For example, an upconversion-linked immunoassay (ULISA) allowed for the sensitive detection of the pharmaceutical diclofenac in environmental water samples. UCNPs can be detected at the single nanoparticle level using a relatively simple wide-field upconversion microscope. In this way, it is possible to implement a single molecule sandwich immunoassay for the detection of diagnostic markers such as prostate-specific antigen (PSA). ER -
GORRIS, Hans-Heiner, Matthias Jürgen MICKERT, Zdeněk FARKA and Antonín HLAVÁČEK. Photon-Upconversion Nanoparticles Give a New Twist to Classic Analytical Tools. In \textit{2017 MRS Fall Meeting \&{} Exhibit}. 2017.
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