J 2017

Effects of polyphenol compounds melanin on NAFLD/NASH prevention

BELEMETS, N., N. KOBYLIAK, O. VIRCHENKO, T. FALALYEYEVA, T. OLENA et. al.

Basic information

Original name

Effects of polyphenol compounds melanin on NAFLD/NASH prevention

Authors

BELEMETS, N. (804 Ukraine), N. KOBYLIAK (804 Ukraine), O. VIRCHENKO (804 Ukraine), T. FALALYEYEVA (804 Ukraine), T. OLENA (804 Ukraine), P. BODNAR (804 Ukraine), O. SAVCHUK (804 Ukraine), T. GALENOVA (804 Ukraine), M. CAPRNDA (703 Slovakia), L. RODRIGO (724 Spain), L. SKLADANY (703 Slovakia), D. DELEV (100 Bulgaria), R. OPATRILOVA (203 Czech Republic), Peter KRUŽLIAK (703 Slovakia, guarantor, belonging to the institution), T. BEREGOVA (804 Ukraine) and L. OSTAPCHENKO (804 Ukraine)

Edition

BIOMEDICINE & PHARMACOTHERAPY, PARIS, ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2017, 0753-3322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.457

RIV identification code

RIV/00216224:14110/17:00098633

Organization unit

Faculty of Medicine

DOI

UT WoS

000395528000031

Keywords in English

Non-alcoholic liver disease (NAFLD); Nonalcoholic steatohepatitis (NASH); Reactive oxygen species (ROS); Inflammation; Cytokines; Therapy; Melanin

Tags

Tags

International impact, Reviewed
Změněno: 20/3/2018 13:02, Soňa Böhmová

Abstract

V originále

Background: One of the pathogenic mechanisms of the progression non-alcoholic liver disease ( NAFLD) to nonalcoholic steatohepatitis (NASH) is the accumulation of reactive oxygen species (ROS). So, antioxidant therapy is necessary for successful treatment of the liver injury. We have paid attention to melanin produced by yeast Nadsoniella nigra strain X-1 as novel antioxidant and anti-inflammatory agents with low toxicity. In current study we aimed to investigate the preventive effect of melanin on the monosodium glutamate (MSG) induced NAFLD model in rats. Methods: The study was carried out on 45 Wistar rats that were divided into 3 groups: intact, MSG-and MSG + melanin groups (n = 15 in each group). Newborn rats of MSG-and MSG + melanin groups were administered with MSG (4 mg/g, 8 mu l/g, subcutaneously) at 2nd-10th days of life. Since the age of 1 month, rats of MSG-group were treated with water (0.25 ml/100 g), rats of MSG + melanin groups-with melanin (1 mg/kg) dissolved in water (0.25 ml/100 g). Introduction: had been performed intermittently (two-week courses alternated with two-week breaks) for 3 months. In 4-month rats anthropometrical parameters and visceral adipose tissue (VAT) mass were estimated. To assess morphological changes in liver we used NAS (NAFLD activity score). The content of pro-inflammatory cytokines (interleukin (IL)-1 beta, IL-12Bp40, interferon (INF)-gamma) and anti-inflammatory cytokines (IL-4, IL-10, tumor growth factor (TGF)-beta) were measured by ELISA. Results: We found significantly lower total score (1.0 +/- 0.19 vs 3.33 +/- 0.36, p < 0.001), degree of steatosis (0.73 +/- 0.18 vs 1.80 +/- 0.17, p < 0.001) and manifestation of lobular inflammation (0.27 +/- 0.11 vs 1.20 +/- 0.17, p < 0.001) due to NAFLD activity score in MSG + melanin group compared to MSG-obesity. NASH we confirmed only in 33.3% of rats with MSG-obesity that was significantly higher than after melanin (6.7%) administration (p = 0.033). Melanin administration reduce amount of visceral fat on 44.5% (p 0.001) as compared to MSG-obesity group. Melanin reduced the content of IL-1 beta in rat serum and restored the level of anti-inflammatory cytokines (IL-10, TGF-beta) to the control values. Conclusion: Thus, the administration of melanin can prevent development of NAFLD/NASH in rats with MSG-induced obesity and can be considered as possible novel therapeutic agents but further studies to confirm its action needed. (C) 2017 Elsevier Masson SAS. All rights reserved.