KOVÁČ, Jozef and Ivan KUSHKEVYCH. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria. Online. In MendelNet 2017 Proceedings of 24th International PhD Students Conference. Brno, Czech Republic: Faculty of AgriSciences, Mendel University in Brno, 2017, p. 702-707. ISBN 978-80-7509-529-9.
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Basic information
Original name New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria
Authors KOVÁČ, Jozef (703 Slovakia, belonging to the institution) and Ivan KUSHKEVYCH (804 Ukraine, guarantor, belonging to the institution).
Edition Brno, Czech Republic, MendelNet 2017 Proceedings of 24th International PhD Students Conference, p. 702-707, 6 pp. 2017.
Publisher Faculty of AgriSciences, Mendel University in Brno
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
WWW URL
RIV identification code RIV/00216224:14310/17:00098646
Organization unit Faculty of Science
ISBN 978-80-7509-529-9
UT WoS 000440194500125
Keywords in English sulfate-reducing bacteria; Desulfovibrio; cultivation media; modification of conditions; bacterial growth
Tags NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: doc. Ivan Kushkevych, Ph.D., učo 238729. Changed: 14/8/2018 10:06.
Abstract
Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.
Links
MUNI/A/0906/2016, interní kód MUName: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 1 (Acronym: SVMIK)
Investor: Masaryk University, Category A
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