New modification of cultivation medium for isolation and growth
of intestinal sulfate-reducing bacteria

D 2017

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

KOVÁČ, Jozef and Ivan KUSHKEVYCH

Basic information

Original name

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

Authors

KOVÁČ, Jozef (703 Slovakia, belonging to the institution) and Ivan KUSHKEVYCH (804 Ukraine, guarantor, belonging to the institution)

Edition

Brno, Czech Republic, MendelNet 2017 Proceedings of 24th International PhD Students Conference, p. 702-707, 6 pp. 2017

Publisher

Faculty of AgriSciences, Mendel University in Brno

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14310/17:00098646

Organization unit

Faculty of Science

ISBN

978-80-7509-529-9

UT WoS

000440194500125

Keywords in English

sulfate-reducing bacteria; Desulfovibrio; cultivation media; modification of conditions; bacterial growth

Abstract

V originále

Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.

Links

MUNI/A/0906/2016, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 1 (Acronym: SVMIK)

Investor: Masaryk University, Category A

Citovat

KOVÁČ, Jozef and Ivan KUSHKEVYCH. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria. Online. In MendelNet 2017 Proceedings of 24th International PhD Students Conference. Brno, Czech Republic: Faculty of AgriSciences, Mendel University in Brno, 2017, p. 702-707. ISBN 978-80-7509-529-9.

@inproceedings{1398314,
   author = {Kováč, Jozef and Kushkevych, Ivan},
   address = {Brno, Czech Republic},
   booktitle = {MendelNet 2017 Proceedings of 24th International PhD Students Conference},
   keywords = {sulfate-reducing bacteria; Desulfovibrio; cultivation media; modification of conditions; bacterial growth},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno, Czech Republic},
   isbn = {978-80-7509-529-9},
   pages = {702-707},
   publisher = {Faculty of AgriSciences, Mendel University in Brno},
   title = {New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria},
   url = {https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf},
   year = {2017}
}

TY  - JOUR
ID  - 1398314
AU  - Kováč, Jozef - Kushkevych, Ivan
PY  - 2017
TI  - New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria
PB  - Faculty of AgriSciences, Mendel University in Brno
CY  - Brno, Czech Republic
SN  - 9788075095299
KW  - sulfate-reducing bacteria
KW  - Desulfovibrio
KW  - cultivation media
KW  - modification of conditions
KW  - bacterial growth
UR  - https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf
L2  - https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf
N2  - Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.
ER  -

KOVÁČ, Jozef and Ivan KUSHKEVYCH. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria. Online. In \textit{MendelNet 2017 Proceedings of 24th International PhD Students Conference}. Brno, Czech Republic: Faculty of AgriSciences, Mendel University in Brno, 2017, p.~702-707. ISBN~978-80-7509-529-9.

Detailed Information on Publication Record