The application of comet assay in monitoring of the
immunosuppression level in a patient with toxic epidermal
necrolysis: A case report

J 2017

The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report

LIPOVÝ, Břetislav, Milena MATEJOVIČOVÁ, H. ŘIHOVÁ, M. ŠTIKAROVSKÁ, Lucie NOVOTNÁ et. al.

Basic information

Original name

The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report

Authors

LIPOVÝ, Břetislav (203 Czech Republic, guarantor, belonging to the institution), Milena MATEJOVIČOVÁ (203 Czech Republic, belonging to the institution), H. ŘIHOVÁ (203 Czech Republic), M. ŠTIKAROVSKÁ (203 Czech Republic), Lucie NOVOTNÁ (203 Czech Republic, belonging to the institution), Miroslava HLAVÁČOVÁ (703 Slovakia, belonging to the institution), Hana PAULOVÁ (203 Czech Republic, belonging to the institution), I. SUCHÁNEK (203 Czech Republic) and Pavel BRYCHTA (203 Czech Republic)

Edition

Burns Open, Amsterdam, Elsevier, 2017, 2468-9122

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/17:00098656

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.burnso.2017.05.007

Keywords in English

Toxic epidermal necrolysis; Immunosuppression; Comet assay

Abstract

V originále

Toxic epidermal necrolysis (TEN) represents the most serious affection within the SCARs group (Severe Cutaneous Adverse Reactions). It is an autoimmune disease manifested by extensive epidermal and mucosal exfoliation. The basic approach in the therapy of patients suffering from toxic epidermal necrolysis is the administration of immunosuppressive medications. Comet assay is a fast, sensitive and relatively easy method used to detect DNA damage levels. We present a case report of a woman with toxic epidermal necrolysis treated with cyclosporine A. During the therapy, comet assay was used to detect the level of DNA damage of circulating lymphocytes and it helped us to reveal qualitative abnormalities in these cells.

Citovat

LIPOVÝ, Břetislav, Milena MATEJOVIČOVÁ, H. ŘIHOVÁ, M. ŠTIKAROVSKÁ, Lucie NOVOTNÁ, Miroslava HLAVÁČOVÁ, Hana PAULOVÁ, I. SUCHÁNEK and Pavel BRYCHTA. The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report. Burns Open. Amsterdam: Elsevier, 2017, vol. 1, No 2, p. 80-83. ISSN 2468-9122. Available from: https://dx.doi.org/10.1016/j.burnso.2017.05.007.

@article{1398340,
   author = {Lipový, Břetislav and Matejovičová, Milena and Řihová, H. and Štikarovská, M. and Novotná, Lucie and Hlaváčová, Miroslava and Paulová, Hana and Suchánek, I. and Brychta, Pavel},
   article_location = {Amsterdam},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.burnso.2017.05.007},
   keywords = {Toxic epidermal necrolysis; Immunosuppression; Comet assay},
   language = {eng},
   issn = {2468-9122},
   journal = {Burns Open},
   title = {The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report},
   volume = {1},
   year = {2017}
}

TY  - JOUR
ID  - 1398340
AU  - Lipový, Břetislav - Matejovičová, Milena - Řihová, H. - Štikarovská, M. - Novotná, Lucie - Hlaváčová, Miroslava - Paulová, Hana - Suchánek, I. - Brychta, Pavel
PY  - 2017
TI  - The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report
JF  - Burns Open
VL  - 1
IS  - 2
SP  - 80-83
EP  - 80-83
PB  - Elsevier
SN  - 24689122
KW  - Toxic epidermal necrolysis
KW  - Immunosuppression
KW  - Comet assay
N2  - Toxic epidermal necrolysis (TEN) represents the most serious affection within the SCARs group (Severe Cutaneous Adverse Reactions). It is an autoimmune disease manifested by extensive epidermal and mucosal exfoliation. The basic approach in the therapy of patients suffering from toxic epidermal necrolysis is the administration of immunosuppressive medications. Comet assay is a fast, sensitive and relatively easy method used to detect DNA damage levels. We present a case report of a woman with toxic epidermal necrolysis treated with cyclosporine A. During the therapy, comet assay was used to detect the level of DNA damage of circulating lymphocytes and it helped us to reveal qualitative abnormalities in these cells.
ER  -

LIPOVÝ, Břetislav, Milena MATEJOVIČOVÁ, H. ŘIHOVÁ, M. ŠTIKAROVSKÁ, Lucie NOVOTNÁ, Miroslava HLAVÁČOVÁ, Hana PAULOVÁ, I. SUCHÁNEK and Pavel BRYCHTA. The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report. \textit{Burns Open}. Amsterdam: Elsevier, 2017, vol.~1, No~2, p.~80-83. ISSN~2468-9122. Available from: https://dx.doi.org/10.1016/j.burnso.2017.05.007.

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