Alamandine reverses hyperhomocysteinemia-induced vascular
dysfunction via PKA-dependent mechanisms

J 2017

Alamandine reverses hyperhomocysteinemia-induced vascular dysfunction via PKA-dependent mechanisms

QARADAKHI, T.., M.T. MATSOUKAS, A. HAYES, E. RYBALKA, M. CAPRNDA et. al.

Basic information

Original name

Alamandine reverses hyperhomocysteinemia-induced vascular dysfunction via PKA-dependent mechanisms

Authors

QARADAKHI, T.., M.T. MATSOUKAS, A. HAYES, E. RYBALKA, M. CAPRNDA, K. RIMAROVA, Milan SEPŠI, D. BUSSELBERG, Peter KRUŽLIAK, J. MATSOUKAS, V. APOSTOLOPOULOS and A. ZULLI

Edition

CARDIOVASCULAR THERAPEUTICS, HOBOKEN, WILEY, 2017, 1755-5914

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30201 Cardiac and Cardiovascular systems

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.245

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/1755-5922.12306

UT WoS

000414962500013

Keywords in English

Alamandine; Endothelial dysfunction; Homocysteine; MrgD; Protein kinase A

Abstract

V originále

IntroductionHyperhomocysteinemia (HHcy) impairs nitric oxide endothelium-dependent vasodilation, consequently leading to atherosclerosis, a risk factor for cardiovascular disease. Novel treatments for HHcy are necessary. AimWe tested the hypothesis that alamandine, a vasoactive peptide of the renin-angiotensin system (RAS), could reverse HHcy-induced vascular dysfunction through the MrgD receptor and that this is mediated by the protein kinase A (PKA) pathway. Furthermore, we sought to determine a putative binding model of alamandine to the MrgD receptor through docking and molecular dynamics simulations. MethodThe abdominal aorta was excised from New Zealand white rabbits (n=15) and incubated with 3 mmol/L Hcy (to mimic HHcy) to induce vascular dysfunction in vitro. Vascular function was assessed by vasodilatory responses to cumulative doses of acetylcholine. ResultVasodilation was significantly impaired in HHcy-incubated aortic rings while alamandine reversed this effect (control, 74.25.0%; Hcy, 30.3 +/- 9.8%; alamandine+Hcy, 59.7 +/- 4.8%, P<.0001). KT5720 (PKA inhibitor) significantly inhibited the ability of alamandine to attenuate the impaired vasodilation caused by HHcy (KT5720+Hcy+alamandine, 27.1 +/- 24.1, P<.01). Following immunohistochemistry analysis, the MrgD receptor was highly expressed within the media and endothelial layer of aortic rings in HHcy compared to control (media: 0.23 +/- 0.003 vs control 0.16 +/- 0.01, P<.05 and endothelium: 0.68 +/- 0.07 vs control 0.13 +/- 0.02, P<.01, in PA/I (A.U) units). Computational studies also propose certain interactions of alamandine within the MrgD transmembrane domain. ConclusionThis study shows that alamandine is effective in reversing HHcy-induced vascular dysfunction, possibly through the PKA signaling pathway via MrgD. Our results indicate a therapeutic potential of alamandine in reversing the detrimental effects of HHcy.

Citovat

QARADAKHI, T.., M.T. MATSOUKAS, A. HAYES, E. RYBALKA, M. CAPRNDA, K. RIMAROVA, Milan SEPŠI, D. BUSSELBERG, Peter KRUŽLIAK, J. MATSOUKAS, V. APOSTOLOPOULOS and A. ZULLI. Alamandine reverses hyperhomocysteinemia-induced vascular dysfunction via PKA-dependent mechanisms. CARDIOVASCULAR THERAPEUTICS. HOBOKEN: WILEY, 2017, vol. 35, No 6, p. 1-11. ISSN 1755-5914. Available from: https://dx.doi.org/10.1111/1755-5922.12306.

@article{1399059,
   author = {Qaradakhi, T.. and Matsoukas, M.T. and Hayes, A. and Rybalka, E. and Caprnda, M. and Rimarova, K. and Sepši, Milan and Busselberg, D. and Kružliak, Peter and Matsoukas, J. and Apostolopoulos, V. and Zulli, A.},
   article_location = {HOBOKEN},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/1755-5922.12306},
   keywords = {Alamandine; Endothelial dysfunction; Homocysteine; MrgD; Protein kinase A},
   language = {eng},
   issn = {1755-5914},
   journal = {CARDIOVASCULAR THERAPEUTICS},
   title = {Alamandine reverses hyperhomocysteinemia-induced vascular dysfunction via PKA-dependent mechanisms},
   url = {http://dx.doi.org/10.1111/1755-5922.12306},
   volume = {35},
   year = {2017}
}

TY  - JOUR
ID  - 1399059
AU  - Qaradakhi, T.. - Matsoukas, M.T. - Hayes, A. - Rybalka, E. - Caprnda, M. - Rimarova, K. - Sepši, Milan - Busselberg, D. - Kružliak, Peter - Matsoukas, J. - Apostolopoulos, V. - Zulli, A.
PY  - 2017
TI  - Alamandine reverses hyperhomocysteinemia-induced vascular dysfunction via PKA-dependent mechanisms
JF  - CARDIOVASCULAR THERAPEUTICS
VL  - 35
IS  - 6
SP  - 1-11
EP  - 1-11
PB  - WILEY
SN  - 17555914
KW  - Alamandine
KW  - Endothelial dysfunction
KW  - Homocysteine
KW  - MrgD
KW  - Protein kinase A
UR  - http://dx.doi.org/10.1111/1755-5922.12306
L2  - http://dx.doi.org/10.1111/1755-5922.12306
N2  - IntroductionHyperhomocysteinemia (HHcy) impairs nitric oxide endothelium-dependent vasodilation, consequently leading to atherosclerosis, a risk factor for cardiovascular disease. Novel treatments for HHcy are necessary. AimWe tested the hypothesis that alamandine, a vasoactive peptide of the renin-angiotensin system (RAS), could reverse HHcy-induced vascular dysfunction through the MrgD receptor and that this is mediated by the protein kinase A (PKA) pathway. Furthermore, we sought to determine a putative binding model of alamandine to the MrgD receptor through docking and molecular dynamics simulations. MethodThe abdominal aorta was excised from New Zealand white rabbits (n=15) and incubated with 3 mmol/L Hcy (to mimic HHcy) to induce vascular dysfunction in vitro. Vascular function was assessed by vasodilatory responses to cumulative doses of acetylcholine. ResultVasodilation was significantly impaired in HHcy-incubated aortic rings while alamandine reversed this effect (control, 74.25.0%; Hcy, 30.3 +/- 9.8%; alamandine+Hcy, 59.7 +/- 4.8%, P<.0001). KT5720 (PKA inhibitor) significantly inhibited the ability of alamandine to attenuate the impaired vasodilation caused by HHcy (KT5720+Hcy+alamandine, 27.1 +/- 24.1, P<.01). Following immunohistochemistry analysis, the MrgD receptor was highly expressed within the media and endothelial layer of aortic rings in HHcy compared to control (media: 0.23 +/- 0.003 vs control 0.16 +/- 0.01, P<.05 and endothelium: 0.68 +/- 0.07 vs control 0.13 +/- 0.02, P<.01, in PA/I (A.U) units). Computational studies also propose certain interactions of alamandine within the MrgD transmembrane domain. ConclusionThis study shows that alamandine is effective in reversing HHcy-induced vascular dysfunction, possibly through the PKA signaling pathway via MrgD. Our results indicate a therapeutic potential of alamandine in reversing the detrimental effects of HHcy.
ER  -

QARADAKHI, T.., M.T. MATSOUKAS, A. HAYES, E. RYBALKA, M. CAPRNDA, K. RIMAROVA, Milan SEPŠI, D. BUSSELBERG, Peter KRUŽLIAK, J. MATSOUKAS, V. APOSTOLOPOULOS and A. ZULLI. Alamandine reverses hyperhomocysteinemia-induced vascular dysfunction via PKA-dependent mechanisms. \textit{CARDIOVASCULAR THERAPEUTICS}. HOBOKEN: WILEY, 2017, vol.~35, No~6, p.~1-11. ISSN~1755-5914. Available from: https://dx.doi.org/10.1111/1755-5922.12306.

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