An improved design to capture magnetic microparticles for
capillary electrophoresis based immobilized microenzyme
reactors

J 2018

An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors

RAMANA, Pranov, Jan SCHEJBAL, Kristof HOUTHOOFD, Johan MARTENS, Adams ERWIN et. al.

Basic information

Original name

An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors

Name in Czech

Vylepšený design pro spojeni CE IMER

Authors

RAMANA, Pranov (56 Belgium), Jan SCHEJBAL (203 Czech Republic, belonging to the institution), Kristof HOUTHOOFD (56 Belgium), Johan MARTENS (56 Belgium), Adams ERWIN (56 Belgium), Patrick AUGUSTIJNS (56 Belgium), Zdeněk GLATZ (203 Czech Republic, belonging to the institution) and Ann VAN SCHEPDAEL (56 Belgium, guarantor)

Edition

Electrophoresis, Německo, WILEY-VCH Verlag GmbH, 2018, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.754

RIV identification code

RIV/00216224:14310/18:00100767

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/elps.201700434

UT WoS

000429417500008

Keywords (in Czech)

IMER; IMER; Magnetické mikročástice; Immobilizace; In-line CE

Keywords in English

IMER; Magnetic microparticles; Immobilization; In-line capillary electrophoresis

Abstract

V originále

In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in-line capillary electrophoresis with clozapine as a model substrate.

Links

GA16-06106S, research and development project

Name: Vysoce efektivní systém založený na kapilární elektroforéze pro screening inhibitorů beta-sekretázy jako terapeutického cíle pro Alzheimerovu chorobu (Acronym: Alzheimer)

Investor: Czech Science Foundation

MUNI/A/1278/2016, interní kód MU

Name: Podpora biochemického výzkumu v roce 2017

Investor: Masaryk University, Category A

Citovat

RAMANA, Pranov, Jan SCHEJBAL, Kristof HOUTHOOFD, Johan MARTENS, Adams ERWIN, Patrick AUGUSTIJNS, Zdeněk GLATZ and Ann VAN SCHEPDAEL. An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors. Electrophoresis. Německo: WILEY-VCH Verlag GmbH, 2018, vol. 2018, No 7, p. 981-988. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201700434.

@article{1400918,
   author = {Ramana, Pranov and Schejbal, Jan and Houthoofd, Kristof and Martens, Johan and Erwin, Adams and Augustijns, Patrick and Glatz, Zdeněk and Van Schepdael, Ann},
   article_location = {Německo},
   article_number = {7},
   doi = {http://dx.doi.org/10.1002/elps.201700434},
   keywords = {IMER; Magnetic microparticles; Immobilization; In-line capillary electrophoresis},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors},
   url = {http://dx.doi.org/10.1002/elps.201700434},
   volume = {2018},
   year = {2018}
}

TY  - JOUR
ID  - 1400918
AU  - Ramana, Pranov - Schejbal, Jan - Houthoofd, Kristof - Martens, Johan - Erwin, Adams - Augustijns, Patrick - Glatz, Zdeněk - Van Schepdael, Ann
PY  - 2018
TI  - An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors
JF  - Electrophoresis
VL  - 2018
IS  - 7
SP  - 981-988
EP  - 981-988
PB  - WILEY-VCH Verlag GmbH
SN  - 01730835
KW  - IMER
KW  - Magnetic microparticles
KW  - Immobilization
KW  - In-line capillary electrophoresis
UR  - http://dx.doi.org/10.1002/elps.201700434
N2  - In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in-line capillary electrophoresis with clozapine as a model substrate.
ER  -

RAMANA, Pranov, Jan SCHEJBAL, Kristof HOUTHOOFD, Johan MARTENS, Adams ERWIN, Patrick AUGUSTIJNS, Zdeněk GLATZ and Ann VAN SCHEPDAEL. An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors. \textit{Electrophoresis}. Německo: WILEY-VCH Verlag GmbH, 2018, vol.~2018, No~7, p.~981-988. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201700434.

Detailed Information on Publication Record