Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA)
feeding on selected markers expression in rats

D 2017

Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats

WIJACKI, Jan, Tomas KOMPRDA, Jana NEUWIRTHOVÁ, Břetislav GÁL, Veronika ROZIKOVA et. al.

Basic information

Original name

Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats

Authors

WIJACKI, Jan (203 Czech Republic), Tomas KOMPRDA (203 Czech Republic), Jana NEUWIRTHOVÁ (203 Czech Republic, guarantor, belonging to the institution), Břetislav GÁL (203 Czech Republic, belonging to the institution) and Veronika ROZIKOVA (203 Czech Republic)

Edition

Brno, MendelNet 2017 : Proceedings of 24th International PhD Students Conference, p. 756-760, 5 pp. 2017

Publisher

Mendel University in Brno

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

[online]

RIV identification code

RIV/00216224:14110/17:00099019

Organization unit

Faculty of Medicine

ISBN

978-80-7509-529-9

UT WoS

000440194500136

Keywords in English

rat; DHA; EPA; fatty acids; RT-PCR

Abstract

V originále

The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.

Citovat

WIJACKI, Jan, Tomas KOMPRDA, Jana NEUWIRTHOVÁ, Břetislav GÁL and Veronika ROZIKOVA. Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats. Online. In Radim Cerkal, Natálie Březinová Belcredi, Lenka Prokešová, Patrik Vacek. MendelNet 2017 : Proceedings of 24th International PhD Students Conference. Brno: Mendel University in Brno, 2017, p. 756-760. ISBN 978-80-7509-529-9.

@inproceedings{1400936,
   author = {Wijacki, Jan and Komprda, Tomas and Neuwirthová, Jana and Gál, Břetislav and Rozikova, Veronika},
   address = {Brno},
   booktitle = {MendelNet 2017 : Proceedings of 24th International PhD Students Conference},
   editor = {Radim Cerkal, Natálie Březinová Belcredi, Lenka Prokešová, Patrik Vacek},
   keywords = {rat; DHA; EPA; fatty acids; RT-PCR},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-7509-529-9},
   pages = {756-760},
   publisher = {Mendel University in Brno},
   title = {Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats},
   url = {https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf},
   year = {2017}
}

TY  - JOUR
ID  - 1400936
AU  - Wijacki, Jan - Komprda, Tomas - Neuwirthová, Jana - Gál, Břetislav - Rozikova, Veronika
PY  - 2017
TI  - Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats
PB  - Mendel University in Brno
CY  - Brno
SN  - 9788075095299
KW  - rat
KW  - DHA
KW  - EPA
KW  - fatty acids
KW  - RT-PCR
UR  - https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf
N2  - The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.
ER  -

WIJACKI, Jan, Tomas KOMPRDA, Jana NEUWIRTHOVÁ, Břetislav GÁL and Veronika ROZIKOVA. Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats. Online. In Radim Cerkal, Natálie Březinová Belcredi, Lenka Prokešová, Patrik Vacek. \textit{MendelNet 2017 : Proceedings of 24th International PhD Students Conference}. Brno: Mendel University in Brno, 2017, p.~756-760. ISBN~978-80-7509-529-9.

Detailed Information on Publication Record