WIJACKI, Jan, Tomas KOMPRDA, Jana NEUWIRTHOVÁ, Břetislav GÁL and Veronika ROZIKOVA. Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats. In Radim Cerkal, Natálie Březinová Belcredi, Lenka Prokešová, Patrik Vacek. MendelNet 2017 : Proceedings of 24th International PhD Students Conference. Brno: Mendel University in Brno. p. 756-760. ISBN 978-80-7509-529-9. 2017.
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Basic information
Original name Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats
Authors WIJACKI, Jan (203 Czech Republic), Tomas KOMPRDA (203 Czech Republic), Jana NEUWIRTHOVÁ (203 Czech Republic, guarantor, belonging to the institution), Břetislav GÁL (203 Czech Republic, belonging to the institution) and Veronika ROZIKOVA (203 Czech Republic).
Edition Brno, MendelNet 2017 : Proceedings of 24th International PhD Students Conference, p. 756-760, 5 pp. 2017.
Publisher Mendel University in Brno
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 30200 3.2 Clinical medicine
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
WWW [online]
RIV identification code RIV/00216224:14110/17:00099019
Organization unit Faculty of Medicine
ISBN 978-80-7509-529-9
UT WoS 000440194500136
Keywords in English rat; DHA; EPA; fatty acids; RT-PCR
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 22/8/2018 11:26.
Abstract
The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.
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