J 2018

Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells

VAŇHARA, Petr, Lukáš KUČERA, Lubomír PROKEŠ, Lucie JUREČKOVÁ, Eladia María PEÑA-MÉNDEZ et. al.

Basic information

Original name

Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells

Authors

VAŇHARA, Petr (203 Czech Republic, belonging to the institution), Lukáš KUČERA (203 Czech Republic, belonging to the institution), Lubomír PROKEŠ (203 Czech Republic, belonging to the institution), Lucie JUREČKOVÁ (203 Czech Republic, belonging to the institution), Eladia María PEÑA-MÉNDEZ (724 Spain), Josef HAVEL (203 Czech Republic, belonging to the institution) and Aleš HAMPL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Stem Cells Translational Medicine, Hoboken, Wiley, 2018, 2157-6564

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.962

RIV identification code

RIV/00216224:14110/18:00100768

Organization unit

Faculty of Medicine

DOI

UT WoS

000419115400012

Keywords in English

cell culture; differentiation; embryonic stem cells; technology; tissue engineering

Tags

Tags

International impact, Reviewed
Změněno: 26/3/2019 10:47, Soňa Böhmová

Abstract

V originále

The stability of in vitro cell cultures is an important issue for any clinical, bio-industrial, or pharmacological use. Embryonic stem cells are pluripotent; consequently, they possess the ability to differentiate into all three germ layers and are inherently prone to respond to differentiation stimuli. However, long-term culture inevitably yields clones that are best adapted to the culture conditions, passaging regimes, or differentiation sensitivity. This cellular plasticity is a major obstacle in the development of bio-industrial or clinical-grade cultures. At present, the quality control of cell cultures is limited by the lack of reliable (epi)genetic or molecular markers or by the focus on a particular type of instability such as karyotype abnormalities or adverse phenotypic traits. Therefore, there is an ongoing need for robust, feasible, and sensitive methods of determining or confirming cell status and for revealing potential divergences from the optimal state. We modeled both intrinsic and extrinsic changes in human embryonic stem cell (hESC) states using different experimental strategies and addressed the changes in cell status by intact cell mass spectrometry fingerprinting. The analysis of spectral fingerprints by methods routinely used in analytical chemistry clearly distinguished the morphologically and biochemically similar populations of hESCs and provided a biomarker-independent tool for the quality control of cell culture

Links

GA15-11707S, research and development project
Name: Centrosomální abnormality u lidských pluripotentních kmenových buněk.
Investor: Czech Science Foundation
LD15144, research and development project
Name: Buněčné a nebuněčné základy pro regeneraci kostí a zubů (Acronym: TissueENG)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1369/2016, interní kód MU
Name: Zdroje pro tkáňové inženýrství 7 (Acronym: TissueENG 7)
Investor: Masaryk University, Category A