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@article{1400984,
author = {Vaňhara, Petr and Kučera, Lukáš and Prokeš, Lubomír and Jurečková, Lucie and PeñaandMéndez, Eladia María and Havel, Josef and Hampl, Aleš},
article_location = {Hoboken},
article_number = {1},
doi = {http://dx.doi.org/10.1002/sctm.17-0107},
keywords = {cell culture; differentiation; embryonic stem cells; technology; tissue engineering},
language = {eng},
issn = {2157-6564},
journal = {Stem Cells Translational Medicine},
title = {Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells},
url = {http://onlinelibrary.wiley.com/doi/10.1002/sctm.17-0107/abstract},
volume = {7},
year = {2018}
}
TY - JOUR
ID - 1400984
AU - Vaňhara, Petr - Kučera, Lukáš - Prokeš, Lubomír - Jurečková, Lucie - Peña-Méndez, Eladia María - Havel, Josef - Hampl, Aleš
PY - 2018
TI - Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells
JF - Stem Cells Translational Medicine
VL - 7
IS - 1
SP - 109-114
EP - 109-114
PB - Wiley
SN - 21576564
KW - cell culture
KW - differentiation
KW - embryonic stem cells
KW - technology
KW - tissue engineering
UR - http://onlinelibrary.wiley.com/doi/10.1002/sctm.17-0107/abstract
N2 - The stability of in vitro cell cultures is an important issue for any clinical, bio-industrial, or pharmacological use. Embryonic stem cells are pluripotent; consequently, they possess the ability to differentiate into all three germ layers and are inherently prone to respond to differentiation stimuli. However, long-term culture inevitably yields clones that are best adapted to the culture conditions, passaging regimes, or differentiation sensitivity. This cellular plasticity is a major obstacle in the development of bio-industrial or clinical-grade cultures. At present, the quality control of cell cultures is limited by the lack of reliable (epi)genetic or molecular markers or by the focus on a particular type of instability such as karyotype abnormalities or adverse phenotypic traits. Therefore, there is an ongoing need for robust, feasible, and sensitive methods of determining or confirming cell status and for revealing potential divergences from the optimal state. We modeled both intrinsic and extrinsic changes in human embryonic stem cell (hESC) states using different experimental strategies and addressed the changes in cell status by intact cell mass spectrometry fingerprinting. The analysis of spectral fingerprints by methods routinely used in analytical chemistry clearly distinguished the morphologically and biochemically similar populations of hESCs and provided a biomarker-independent tool for the quality control of cell culture
ER -
VAŇHARA, Petr, Lukáš KUČERA, Lubomír PROKEŠ, Lucie JUREČKOVÁ, Eladia María PEÑA-MÉNDEZ, Josef HAVEL and Aleš HAMPL. Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells. \textit{Stem Cells Translational Medicine}. Hoboken: Wiley, 2018, vol.~7, No~1, p.~109-114. ISSN~2157-6564. Available from: https://dx.doi.org/10.1002/sctm.17-0107.
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