A preliminary study of endocannabinoid system regulation in
psychosis: Distinct alterations of CNR1 promoter DNA
methylation in patients with schizophrenia

J 2017

A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia

ADDARIO, D., Vincenzo MICALE, M. DI BARTOLOMEO, Tibor ŠTARK, M. PUCCI et. al.

Basic information

Original name

A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia

Authors

ADDARIO, D. (380 Italy), Vincenzo MICALE (380 Italy, belonging to the institution), M. DI BARTOLOMEO (380 Italy), Tibor ŠTARK (703 Slovakia, guarantor, belonging to the institution), M. PUCCI (380 Italy), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution), M. PALAZZO (380 Italy), Zuzana BABINSKÁ (703 Slovakia, belonging to the institution), L. CREMASCHI (380 Italy), F. DRAGO (380 Italy), C.A. ALTAMURA (380 Italy), M. MACCARRONE (380 Italy) and B. DELL'OSSO (380 Italy)

Edition

Schizophrenia Research, AMSTERDAM, ELSEVIER SCIENCE BV, 2017, 0920-9964

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30215 Psychiatry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.958

RIV identification code

RIV/00216224:14110/17:00099040

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.schres.2017.01.022

UT WoS

000415217800021

Keywords in English

Endocannabinoid system (ECS); Cannabinoid receptor type-1; DNA methylation; Schizophrenia; Methylazoxymethanol (MAM) rat model

Abstract

V originále

Compelling evidence supports the involvement of the endocannabinoid system(ECS) in psychosis vulnerability. We here evaluated the transcriptional regulation of ECS components in human peripheral blood mononuclear cells (PBMCs) obtained from subjects suffering from bipolar disorder, major depressive disorder and schizophrenia, focusing in particular on the effects of DNA methylation. We observed selective alterations of DNA methylation at the promoter of CNR1, the gene coding for the type-1 cannabinoid receptor, in schizophrenic patients (N = 25) with no changes in any other disorder. We confirmed the regulation of CNR1 in a well-validated animal model of schizophrenia, induced by prenatal methylazoxymethanol (MAM) acetate exposure (N = 7 per group) where we found, in the prefrontal cortex, a significant increase in CNR1 expression and a consistent reduction in DNA methylation at specific CpG sites of gene promoter. Overall, our findings suggest a selective dysregulation of ECS in psychosis, and highlight the evaluation of CNR1 DNA methylation levels in PBMCs as a potential biomarker for schizophrenia. (C) 2017 Elsevier B.V. All rights reserved.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1063/2016, interní kód MU

Name: Experimentální a translační farmakologický výzkum a vývoj

Investor: Masaryk University, Category A

Citovat

ADDARIO, D., Vincenzo MICALE, M. DI BARTOLOMEO, Tibor ŠTARK, M. PUCCI, Alexandra ŠULCOVÁ, M. PALAZZO, Zuzana BABINSKÁ, L. CREMASCHI, F. DRAGO, C.A. ALTAMURA, M. MACCARRONE and B. DELL'OSSO. A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia. Schizophrenia Research. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, vol. 188, OCT 2017, p. 132-140. ISSN 0920-9964. Available from: https://dx.doi.org/10.1016/j.schres.2017.01.022.

@article{1400993,
   author = {Addario, D. and Micale, Vincenzo and Di Bartolomeo, M. and Štark, Tibor and Pucci, M. and Šulcová, Alexandra and Palazzo, M. and Babinská, Zuzana and Cremaschi, L. and Drago, F. and Altamura, C.A. and Maccarrone, M. and Dell'Osso, B.},
   article_location = {AMSTERDAM},
   article_number = {OCT 2017},
   doi = {http://dx.doi.org/10.1016/j.schres.2017.01.022},
   keywords = {Endocannabinoid system (ECS); Cannabinoid receptor type-1; DNA methylation; Schizophrenia; Methylazoxymethanol (MAM) rat model},
   language = {eng},
   issn = {0920-9964},
   journal = {Schizophrenia Research},
   title = {A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia},
   volume = {188},
   year = {2017}
}

TY  - JOUR
ID  - 1400993
AU  - Addario, D. - Micale, Vincenzo - Di Bartolomeo, M. - Štark, Tibor - Pucci, M. - Šulcová, Alexandra - Palazzo, M. - Babinská, Zuzana - Cremaschi, L. - Drago, F. - Altamura, C.A. - Maccarrone, M. - Dell'Osso, B.
PY  - 2017
TI  - A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia
JF  - Schizophrenia Research
VL  - 188
IS  - OCT 2017
SP  - 132-140
EP  - 132-140
PB  - ELSEVIER SCIENCE BV
SN  - 09209964
KW  - Endocannabinoid system (ECS)
KW  - Cannabinoid receptor type-1
KW  - DNA methylation
KW  - Schizophrenia
KW  - Methylazoxymethanol (MAM) rat model
N2  - Compelling evidence supports the involvement of the endocannabinoid system(ECS) in psychosis vulnerability. We here evaluated the transcriptional regulation of ECS components in human peripheral blood mononuclear cells (PBMCs) obtained from subjects suffering from bipolar disorder, major depressive disorder and schizophrenia, focusing in particular on the effects of DNA methylation. We observed selective alterations of DNA methylation at the promoter of CNR1, the gene coding for the type-1 cannabinoid receptor, in schizophrenic patients (N = 25) with no changes in any other disorder. We confirmed the regulation of CNR1 in a well-validated animal model of schizophrenia, induced by prenatal methylazoxymethanol (MAM) acetate exposure (N = 7 per group) where we found, in the prefrontal cortex, a significant increase in CNR1 expression and a consistent reduction in DNA methylation at specific CpG sites of gene promoter. Overall, our findings suggest a selective dysregulation of ECS in psychosis, and highlight the evaluation of CNR1 DNA methylation levels in PBMCs as a potential biomarker for schizophrenia. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

ADDARIO, D., Vincenzo MICALE, M. DI BARTOLOMEO, Tibor ŠTARK, M. PUCCI, Alexandra ŠULCOVÁ, M. PALAZZO, Zuzana BABINSKÁ, L. CREMASCHI, F. DRAGO, C.A. ALTAMURA, M. MACCARRONE and B. DELL'OSSO. A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia. \textit{Schizophrenia Research}. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, vol.~188, OCT 2017, p.~132-140. ISSN~0920-9964. Available from: https://dx.doi.org/10.1016/j.schres.2017.01.022.

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