Sub-chronic inhalation of lead oxide nanoparticles revealed
their broad distribution and tissue-specific subcellular
localization in target organs

J 2017

Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs

DUMKOVÁ, Jana, T. SMUTNÁ, Lucie VRLÍKOVÁ, Philippe LE COUSTUMER, Z. VEČEŘA et. al.

Basic information

Original name

Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs

Authors

DUMKOVÁ, Jana (203 Czech Republic, belonging to the institution), T. SMUTNÁ (203 Czech Republic), Lucie VRLÍKOVÁ (203 Czech Republic), Philippe LE COUSTUMER (250 France), Z. VEČEŘA (203 Czech Republic), B. DOČEKAL (203 Czech Republic), P. MIKUŠKA (203 Czech Republic), L. ČAPKA (203 Czech Republic), P. FICTUM (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution) and marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution)

Edition

Particle and Fibre Toxicology, London, Biomed Central, 2017, 1743-8977

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.105

RIV identification code

RIV/00216224:14310/17:00099051

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/s12989-017-0236-y

UT WoS

000418716600001

Keywords in English

nanoparticles; lead oxide; electron microscopy; toxicity; inhalation; lung; liver; kidney; spleen; brain

Abstract

V originále

Background: Lead is well known environmental pollutant, which can cause toxic effects in multiple organ systems. However, the influence of lead oxide nanoparticles, frequently emitted to the environment by high temperature technological processes, is still concealed. Therefore, we investigate lead oxide nanoparticle distribution through the body upon their entry into lungs and determine the microscopic and ultramicroscopic changes caused by the nanoparticles in primary and secondary target organs. Methods: Adult female mice (ICR strain) were continuously exposed to lead oxide nanoparticles (PbO-NPs) with an average concentration approximately 106 particles/cm3 for 6 weeks (24 h/day, 7 days/week). At the end of the exposure period, lung, brain, liver, kidney, spleen, and blood were collected for chemical, histological, immunohistochemical and electron microscopic analyses. Results: Lead content was found to be the highest in the kidney and lungs, followed by the liver and spleen; the smallest content of lead was found in brain. Nanoparticles were located in all analysed tissues and their highest number was found in the lung and liver. Kidney, spleen and brain contained lower number of nanoparticles, being about the same in all three organs. Lungs of animals exposed to lead oxide nanoparticles exhibited hyperaemia, small areas of atelectasis, alveolar emphysema, focal acute catarrhal bronchiolitis and also haemostasis with presence of siderophages in some animals. Nanoparticles were located in phagosomes or formed clusters within cytoplasmic vesicles. In the liver, lead oxide nanoparticle exposure caused hepatic remodeling with enlargement and hydropic degeneration of hepatocytes, centrilobular hypertrophy of hepatocytes with karyomegaly, areas of hepatic necrosis, occasional periportal inflammation, and extensive accumulation of lipid droplets. Nanoparticles were accumulated within mitochondria and peroxisomes forming aggregates enveloped by an electron-dense mitochondrial matrix. Only in some kidney samples, we observed areas of inflammatory infiltrates around renal corpuscles, tubules or vessels in the cortex. Lead oxide nanoparticles were dispersed in the cytoplasm, but not within cell organelles. There were no significant morphological changes in the spleen as a secondary target organ. Thus, pathological changes correlated with the amount of nanoparticles found in cells rather than with the concentration of lead in a given organ. Conclusions: Sub-chronic exposure to lead oxide nanoparticles has profound negative effects at both cellular and tissue levels. Notably, the fate and arrangement of lead oxide nanoparticles were dependent on the type of organs.

Citovat

DUMKOVÁ, Jana, T. SMUTNÁ, Lucie VRLÍKOVÁ, Philippe LE COUSTUMER, Z. VEČEŘA, B. DOČEKAL, P. MIKUŠKA, L. ČAPKA, P. FICTUM, Aleš HAMPL and marcela BUCHTOVÁ. Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs. Particle and Fibre Toxicology. London: Biomed Central, 2017, vol. 14, December, p. nestránkováno, 19 pp. ISSN 1743-8977. Available from: https://dx.doi.org/10.1186/s12989-017-0236-y.

@article{1401051,
   author = {Dumková, Jana and Smutná, T. and Vrlíková, Lucie and Le Coustumer, Philippe and Večeřa, Z. and Dočekal, B. and Mikuška, P. and Čapka, L. and Fictum, P. and Hampl, Aleš and Buchtová, marcela},
   article_location = {London},
   article_number = {December},
   doi = {http://dx.doi.org/10.1186/s12989-017-0236-y},
   keywords = {nanoparticles; lead oxide; electron microscopy; toxicity; inhalation; lung; liver; kidney; spleen; brain},
   language = {eng},
   issn = {1743-8977},
   journal = {Particle and Fibre Toxicology},
   title = {Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs},
   url = {https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-017-0236-y},
   volume = {14},
   year = {2017}
}

TY  - JOUR
ID  - 1401051
AU  - Dumková, Jana - Smutná, T. - Vrlíková, Lucie - Le Coustumer, Philippe - Večeřa, Z. - Dočekal, B. - Mikuška, P. - Čapka, L. - Fictum, P. - Hampl, Aleš - Buchtová, marcela
PY  - 2017
TI  - Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs
JF  - Particle and Fibre Toxicology
VL  - 14
IS  - December
SP  - nestránkováno
EP  - nestránkováno
PB  - Biomed Central
SN  - 17438977
KW  - nanoparticles
KW  - lead oxide
KW  - electron microscopy
KW  - toxicity
KW  - inhalation
KW  - lung
KW  - liver
KW  - kidney
KW  - spleen
KW  - brain
UR  - https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-017-0236-y
L2  - https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-017-0236-y
N2  - Background: Lead is well known environmental pollutant, which can cause toxic effects in multiple organ systems. However, the influence of lead oxide nanoparticles, frequently emitted to the environment by high temperature technological processes, is still concealed. Therefore, we investigate lead oxide nanoparticle distribution through the body upon their entry into lungs and determine the microscopic and ultramicroscopic changes caused by the nanoparticles in primary and secondary target organs. Methods: Adult female mice (ICR strain) were continuously exposed to lead oxide nanoparticles (PbO-NPs) with an average concentration approximately 106 particles/cm3 for 6 weeks (24 h/day, 7 days/week). At the end of the exposure period, lung, brain, liver, kidney, spleen, and blood were collected for chemical, histological, immunohistochemical and electron microscopic analyses. Results: Lead content was found to be the highest in the kidney and lungs, followed by the liver and spleen; the smallest content of lead was found in brain. Nanoparticles were located in all analysed tissues and their highest number was found in the lung and liver. Kidney, spleen and brain contained lower number of nanoparticles, being about the same in all three organs. Lungs of animals exposed to lead oxide nanoparticles exhibited hyperaemia, small areas of atelectasis, alveolar emphysema, focal acute catarrhal bronchiolitis and also haemostasis with presence of siderophages in some animals. Nanoparticles were located in phagosomes or formed clusters within cytoplasmic vesicles. In the liver, lead oxide nanoparticle exposure caused hepatic remodeling with enlargement and hydropic degeneration of hepatocytes, centrilobular hypertrophy of hepatocytes with karyomegaly, areas of hepatic necrosis, occasional periportal inflammation, and extensive accumulation of lipid droplets. Nanoparticles were accumulated within mitochondria and peroxisomes forming aggregates enveloped by an electron-dense mitochondrial matrix. Only in some kidney samples, we observed areas of inflammatory infiltrates around renal corpuscles, tubules or vessels in the cortex. Lead oxide nanoparticles were dispersed in the cytoplasm, but not within cell organelles. There were no significant morphological changes in the spleen as a secondary target organ. Thus, pathological changes correlated with the amount of nanoparticles found in cells rather than with the concentration of lead in a given organ. Conclusions: Sub-chronic exposure to lead oxide nanoparticles has profound negative effects at both cellular and tissue levels. Notably, the fate and arrangement of lead oxide nanoparticles were dependent on the type of organs.
ER  -

DUMKOVÁ, Jana, T. SMUTNÁ, Lucie VRLÍKOVÁ, Philippe LE COUSTUMER, Z. VEČEŘA, B. DOČEKAL, P. MIKUŠKA, L. ČAPKA, P. FICTUM, Aleš HAMPL and marcela BUCHTOVÁ. Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs. \textit{Particle and Fibre Toxicology}. London: Biomed Central, 2017, vol.~14, December, p.~nestránkováno, 19 pp. ISSN~1743-8977. Available from: https://dx.doi.org/10.1186/s12989-017-0236-y.

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