Detailed Information on Publication Record
2017
Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins
NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ and Ondrej ŠEDOBasic information
Original name
Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins
Authors
NEMEC, Alexandr (203 Czech Republic), Lenka RADOLFOVÁ-KŘÍŽOVÁ (203 Czech Republic), Martina MAIXNEROVÁ (203 Czech Republic) and Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution)
Edition
International Journal of Systematic and Evolutionary Microbiology, Society for General Microbiology, 2017, 1466-5026
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.932
RIV identification code
RIV/00216224:14740/17:00095314
Organization unit
Central European Institute of Technology
UT WoS
000408263300010
Keywords in English
gyrB gene; MALDI-TOF MS; metabolic and physiological properties; rpoB gene; whole-genome sequence
Tags
International impact, Reviewed
Změněno: 2/3/2018 09:37, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Strains of the genus Acinetobacter, classified as genomic species 13BJ/14TU have been previously associated with human infections and resistance to colistin. To clarify the taxonomy of this provisional group, we investigated 24 strains that have been isolated from humans since the 1960s in 10 countries. The genus-wide analysis of the rpoB and gyrB sequences of all strains and whole-genome sequences of strains representing different rpoB/gyrB genotypes showed that the 24 strains formed a distinct monophyletic group within the so-called haemolytic clade of the genus Acinetobacter. The distinctness of the group at the species level was supported by the results of the cluster analysis of the whole-cell protein fingerprints generated by matrix-assisted laser desorption ionization-time-of-flight MS. The 24 strains had very similar metabolic features and could be distinguished from other members of the genus by the combination of strong haemolytic and proteolytic activities and the ability to oxidize D-glucose and grow on phenylacetate and/or L-phenylalanine. The minimum inhibitory concentrations of the 24 strains to colistin and polymyxin B ranged from 16 to 64 mgl(-1) and from 4 to 32 mgl(-1), respectively, so uniformly reaching the current clinical resistance breakpoint (4mg l(-1)) for these drugs. Genus-wide comparison revealed that such a consistently high level of resistance to polymyxins is a unique feature among species of the genus Acinetobacter, which occur in humans. We conclude that genomic species 13BJ/14TU represents a biologically meaningful and medically relevant species, for which the name Acinetobacter colistiniresistens sp. nov. is proposed. The type strain is NIPH 2036(T) (=CCM 8641(T)=CIP 110478(T)=CCUG 67966(T)=CNCTC 7573(T)).
Links
| GA13-26693S, research and development project |
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| LM2015043, research and development project |
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