| NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ and Ondrej ŠEDO. Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins. International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology, 2017, vol. 67, No 7, p. 2134-2141. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.001903. |
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@article{1401137,
author = {Nemec, Alexandr and RadolfováandKřížová, Lenka and Maixnerová, Martina and Šedo, Ondrej},
article_number = {7},
doi = {http://dx.doi.org/10.1099/ijsem.0.001903},
keywords = {gyrB gene; MALDI-TOF MS; metabolic and physiological properties; rpoB gene; whole-genome sequence},
language = {eng},
issn = {1466-5026},
journal = {International Journal of Systematic and Evolutionary Microbiology},
title = {Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins},
url = {http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001903#tab2},
volume = {67},
year = {2017}
}
TY - JOUR
ID - 1401137
AU - Nemec, Alexandr - Radolfová-Křížová, Lenka - Maixnerová, Martina - Šedo, Ondrej
PY - 2017
TI - Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins
JF - International Journal of Systematic and Evolutionary Microbiology
VL - 67
IS - 7
SP - 2134-2141
EP - 2134-2141
PB - Society for General Microbiology
SN - 14665026
KW - gyrB gene
KW - MALDI-TOF MS
KW - metabolic and physiological properties
KW - rpoB gene
KW - whole-genome sequence
UR - http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001903#tab2
L2 - http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001903#tab2
N2 - Strains of the genus Acinetobacter, classified as genomic species 13BJ/14TU have been previously associated with human infections and resistance to colistin. To clarify the taxonomy of this provisional group, we investigated 24 strains that have been isolated from humans since the 1960s in 10 countries. The genus-wide analysis of the rpoB and gyrB sequences of all strains and whole-genome sequences of strains representing different rpoB/gyrB genotypes showed that the 24 strains formed a distinct monophyletic group within the so-called haemolytic clade of the genus Acinetobacter. The distinctness of the group at the species level was supported by the results of the cluster analysis of the whole-cell protein fingerprints generated by matrix-assisted laser desorption ionization-time-of-flight MS. The 24 strains had very similar metabolic features and could be distinguished from other members of the genus by the combination of strong haemolytic and proteolytic activities and the ability to oxidize D-glucose and grow on phenylacetate and/or L-phenylalanine. The minimum inhibitory concentrations of the 24 strains to colistin and polymyxin B ranged from 16 to 64 mgl(-1) and from 4 to 32 mgl(-1), respectively, so uniformly reaching the current clinical resistance breakpoint (4mg l(-1)) for these drugs. Genus-wide comparison revealed that such a consistently high level of resistance to polymyxins is a unique feature among species of the genus Acinetobacter, which occur in humans. We conclude that genomic species 13BJ/14TU represents a biologically meaningful and medically relevant species, for which the name Acinetobacter colistiniresistens sp. nov. is proposed. The type strain is NIPH 2036(T) (=CCM 8641(T)=CIP 110478(T)=CCUG 67966(T)=CNCTC 7573(T)).
ER -
NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ and Ondrej ŠEDO. Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins. \textit{International Journal of Systematic and Evolutionary Microbiology}. Society for General Microbiology, 2017, vol.~67, No~7, p.~2134-2141. ISSN~1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.001903.
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