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@article{1401784,
author = {Šimara, Pavel and Tesařová, Lenka and Řeháková, Daniela and Farkaš, Šimon and Šalingová, Barbara and Kutálková, Kateřina and Vavrečková, Eva and Matula, Pavel and Matula, Petr and Veverková, Lenka and Krontorád Koutná, Irena},
article_number = {1},
doi = {http://dx.doi.org/10.1089/scd.2017.0132},
keywords = {induced pluripotent stem cells; endothelial differentiation; peripheral blood mononuclear cells},
language = {eng},
issn = {1547-3287},
journal = {Stem Cells and Development},
title = {Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells.},
url = {http://online.liebertpub.com/doi/10.1089/scd.2017.0132},
volume = {27},
year = {2018}
}
TY - JOUR
ID - 1401784
AU - Šimara, Pavel - Tesařová, Lenka - Řeháková, Daniela - Farkaš, Šimon - Šalingová, Barbara - Kutálková, Kateřina - Vavrečková, Eva - Matula, Pavel - Matula, Petr - Veverková, Lenka - Krontorád Koutná, Irena
PY - 2018
TI - Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells.
JF - Stem Cells and Development
VL - 27
IS - 1
SP - 10-22
EP - 10-22
PB - Mary Ann Liebert, Inc. publishers
SN - 15473287
KW - induced pluripotent stem cells
KW - endothelial differentiation
KW - peripheral blood mononuclear cells
UR - http://online.liebertpub.com/doi/10.1089/scd.2017.0132
L2 - http://online.liebertpub.com/doi/10.1089/scd.2017.0132
N2 - New approaches in regenerative medicine and vasculogenesis have generated a demand for sufficient numbers of human endothelial cells (ECs). ECs and their progenitors reside on the interior surface of blood and lymphatic vessels or circulate in peripheral blood; however, their numbers are limited, and they are difficult to expand after isolation. Recent advances in human induced pluripotent stem cell (hiPSC) research have opened possible avenues to generate unlimited numbers of ECs from easily accessible cell sources, such as the peripheral blood. In this study, we reprogrammed peripheral blood mononuclear cells, human umbilical vein endothelial cells (HUVECs), and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. The phenotype profiles, functionality, and genome stability of all hiPSC-derived ECs were assessed and compared with HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts, as shown by the expression of the endothelial surface markers CD31 and CD144 and the results of the functional analysis. Higher expression of endothelial progenitor markers CD34 and kinase insert domain receptor (KDR) was measured in hiPSC-derived ECs. An analysis of phosphorylated histone H2AX (gamma-H2AX) foci revealed that an increased number of DNA double-strand breaks upon reprogramming into pluripotent cells. However, differentiation into ECs restored a normal number of gamma-H2AX foci. Our hiPSCs retained a normal karyotype, with the exception of the HSVEC-derived hiPSC line, which displayed mosaicism due to a gain of chromosome 1. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage. hiPSC-derived ECs are fully functional and comparable to natural ECs. The protocol is eligible for clinical applications in regenerative medicine, if the genomic stability of the pluripotent cell stage is closely monitored.
ER -
ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA, Lenka VEVERKOVÁ and Irena KRONTORÁD KOUTNÁ. Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells. \textit{Stem Cells and Development}. Mary Ann Liebert, Inc. publishers, vol.~27, No~1, p.~10-22. ISSN~1547-3287. doi:10.1089/scd.2017.0132. 2018.
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