KNAPP, Michal, Pavel DOBEŠ, Michal ŘEŘICHA and Pavel HYRŠL. Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods. European Journal of Entomology. AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, vol. 115, No 1, p. 1-6. ISSN 1802-8829. Available from: https://dx.doi.org/10.14411/eje.2018.001.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
Name in Czech Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
Authors KNAPP, Michal (203 Czech Republic), Pavel DOBEŠ (203 Czech Republic, guarantor, belonging to the institution), Michal ŘEŘICHA (203 Czech Republic) and Pavel HYRŠL (203 Czech Republic, belonging to the institution).
Edition European Journal of Entomology, AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, 1802-8829.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.965
RIV identification code RIV/00216224:14310/18:00102177
Organization unit Faculty of Science
Doi http://dx.doi.org/10.14411/eje.2018.001
UT WoS 000434150700001
Keywords (in Czech) Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invazivní druh; hemolymfa; antimikrobiální aktivita; koncentrace hemocytů; koncenttrace proteinů; reflexní krvácení; metoda vzorkování
Keywords in English Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invasive species; haemolymph; antimicrobial activity; haemocyte concentration; protein concentration; reflex bleeding; sampling technique
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavel Dobeš, Ph.D., učo 150960. Changed: 14/10/2018 15:24.
Abstract
Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.
Abstract (in Czech)
Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.
PrintDisplayed: 19/7/2024 17:38