Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods

KNAPP, Michal, Pavel DOBEŠ, Michal ŘEŘICHA and Pavel HYRŠL. Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods. European Journal of Entomology. AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, vol. 115, No 1, p. 1-6. ISSN 1802-8829. Available from: https://dx.doi.org/10.14411/eje.2018.001.

Basic information

Original name

Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods

Name in Czech

Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods

Authors

KNAPP, Michal (203 Czech Republic), Pavel DOBEŠ (203 Czech Republic, guarantor, belonging to the institution), Michal ŘEŘICHA (203 Czech Republic) and Pavel HYRŠL (203 Czech Republic, belonging to the institution)

Edition

European Journal of Entomology, AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, 1802-8829

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.965

RIV identification code

RIV/00216224:14310/18:00102177

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.14411/eje.2018.001

UT WoS

000434150700001

Keywords (in Czech)

Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invazivní druh; hemolymfa; antimikrobiální aktivita; koncentrace hemocytů; koncenttrace proteinů; reflexní krvácení; metoda vzorkování

Keywords in English

Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invasive species; haemolymph; antimicrobial activity; haemocyte concentration; protein concentration; reflex bleeding; sampling technique

Tags

International impact, Reviewed

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Abstract

V originále

Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.

In Czech

Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.