J 2018

Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells.

TYLICHOVÁ, Zuzana, Josef SLAVÍK, Miroslav CIGANEK, Petra OVESNÁ, Pavel KRČMÁŘ et. al.

Basic information

Original name

Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells.

Authors

TYLICHOVÁ, Zuzana (203 Czech Republic, belonging to the institution), Josef SLAVÍK (203 Czech Republic), Miroslav CIGANEK (203 Czech Republic, belonging to the institution), Petra OVESNÁ (203 Czech Republic, belonging to the institution), Pavel KRČMÁŘ (203 Czech Republic), Nicol STRAKOVÁ (203 Czech Republic, belonging to the institution), Miroslav MACHALA (203 Czech Republic, belonging to the institution), Alois KOZUBÍK (203 Czech Republic, belonging to the institution), Jiřina HOFMANOVÁ (203 Czech Republic, belonging to the institution) and Jan VONDRÁČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF CELLULAR BIOCHEMISTRY, Spojené státy americké, Wiley, 2018, 0730-2312

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.448

RIV identification code

RIV/00216224:14310/18:00102187

Organization unit

Faculty of Science

DOI

UT WoS

000430667200041

Keywords in English

butyrate; ceramides; colon cancer; docosahexaenoic acid; lipid analyses; phospholipids

Tags

Tags

International impact, Reviewed
Změněno: 23/4/2024 10:53, Mgr. Michal Petr

Abstract

V originále

Docosahexaenoic acid (DHA) and sodium butyrate (NaBt) exhibit a number of interactive effects on colon cancer cell growth, differentiation, or apoptosis; however, the molecular mechanisms responsible for these interactions and their impact on cellular lipidome are still not fully clear. Here, we show that both dietary agents together induce dynamic alterations of lipid metabolism, specific cellular lipid classes, and fatty acid composition. In HT-29 cell line, a model of differentiating colon carcinoma cells, NaBt supported incorporation of free DHA into non-polar lipids and their accumulation in cytoplasmic lipid droplets. DHA itself was not incorporated into sphingolipids; however, it significantly altered representation of individual ceramide (Cer) classes, in particular in combination with NaBt (DHA/NaBt). We observed altered expression of enzymes involved in Cer metabolism in cells treated with NaBt or DHA/NaBt, and exogenous Cer 16:0 was found to promote induction of apoptosis in differentiating HT-29 cells. NaBt, together with DHA, increased n-3 fatty acid synthesis and attenuated metabolism of monounsaturated fatty acids. Finally, DHA and/or NaBt altered expression of proteins involved in synthesis of fatty acids, including elongase 5, stearoyl CoA desaturase 1, or fatty acid synthase, with NaBt increasing expression of caveolin-1 and CD36 transporter, which may further promote DHA incorporation and its impact on cellular lipidome. In conclusion, our results indicate that interactions of DHA and NaBt exert complex changes in cellular lipidome, which may contribute to the alterations of colon cancer cell differentiation/apoptotic responses. The present data extend our knowledge about the nature of interactive effects of dietary fatty acids.