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@article{1403838,
author = {Kotík, Martin and Vaňáček, Pavel and Kunka, Antonín and Prokop, Zbyněk and Damborský, Jiří},
article_location = {New York},
article_number = {16},
doi = {http://dx.doi.org/10.1007/s00253-017-8393-3},
keywords = {Haloalkane dehalogenase; Metagenomic DNA; Heterologous production; Substrate specificity; Protein stability},
language = {eng},
issn = {0175-7598},
journal = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
title = {Metagenome-derived Haloalkane Dehalogenases with Novel Catalytic Properties},
url = {https://loschmidt.chemi.muni.cz/peg/publications/metagenome-derived-haloalkane-dehalogenases-with-novel-catalytic-properties/},
volume = {101},
year = {2017}
}
TY - JOUR
ID - 1403838
AU - Kotík, Martin - Vaňáček, Pavel - Kunka, Antonín - Prokop, Zbyněk - Damborský, Jiří
PY - 2017
TI - Metagenome-derived Haloalkane Dehalogenases with Novel Catalytic Properties
JF - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
VL - 101
IS - 16
SP - 6385-6397
EP - 6385-6397
PB - Springer
SN - 01757598
KW - Haloalkane dehalogenase
KW - Metagenomic DNA
KW - Heterologous production
KW - Substrate specificity
KW - Protein stability
UR - https://loschmidt.chemi.muni.cz/peg/publications/metagenome-derived-haloalkane-dehalogenases-with-novel-catalytic-properties/
N2 - Haloalkane dehalogenases (HLDs) are environmentally relevant enzymes cleaving a carbon-halogen bond in a wide range of halogenated pollutants. PCR with degenerate primers and genome-walking was used for the retrieval of four HLD-encoding genes from groundwater-derived environmental DNA. Using specific primers and the environmental DNA as a template, we succeeded in generating additional amplicons, resulting altogether in three clusters of sequences with each cluster comprising 8–13 closely related putative HLD-encoding genes. A phylogenetic analysis of the translated genes revealed that three HLDs are members of the HLD-I subfamily, whereas one gene encodes an enzyme from the subfamily HLD-II. Two metagenome-derived HLDs, eHLDB and eHLD-C, each from a different subfamily, were heterologously produced in active form, purified and characterized in terms of their thermostability, pH and temperature optimum, quaternary structure, substrate specificity towards 30 halogenated compounds, and enantioselectivity. eHLD-B and eHLD-C showed striking differences in their activities, substrate preferences, and tolerance to temperature. Profound differences were also determined in the enantiopreference and enantioselectivity of these enzymes towards selected substrates. Comparing our data with those of known HLDs revealed that eHLD-C exhibits a unique combination of high thermostability, high activity, and an unusually broad pH optimum, which covers the entire range of pH 5.5–8.9. Moreover, a so far unreported high thermostability for HLDs was determined for this enzyme at pH values lower than 6.0. Thus, eHLD-C represents an attractive and novel biocatalyst for biotechnological applications.
ER -
KOTÍK, Martin, Pavel VAŇÁČEK, Antonín KUNKA, Zbyněk PROKOP and Jiří DAMBORSKÝ. Metagenome-derived Haloalkane Dehalogenases with Novel Catalytic Properties. \textit{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}. New York: Springer, 2017, vol.~101, No~16, p.~6385-6397. ISSN~0175-7598. Available from: https://dx.doi.org/10.1007/s00253-017-8393-3.
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