FireProt: Web Server for Automated Design of Thermostable
Proteins

J 2017

FireProt: Web Server for Automated Design of Thermostable Proteins

MUSIL, Miloš, Jan ŠTOURAČ, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP et. al.

Basic information

Original name

FireProt: Web Server for Automated Design of Thermostable Proteins

Authors

MUSIL, Miloš (203 Czech Republic, belonging to the institution), Jan ŠTOURAČ (203 Czech Republic, belonging to the institution), Jaroslav BENDL (203 Czech Republic, belonging to the institution), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Jaroslav ZENDULKA (203 Czech Republic), Tomáš MARTÍNEK (203 Czech Republic, belonging to the institution), David BEDNÁŘ (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nucleic Acids Research, Oxford, Oxford University Press, 2017, 0305-1048

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.561

RIV identification code

RIV/00216224:14310/17:00095410

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1093/nar/gkx285

UT WoS

000404427000059

Keywords in English

MULTIPLE SEQUENCE ALIGNMENTS; CORRELATED MUTATIONS; STABILITY CHANGES; EVOLUTIONARY CONSERVATION; HALOALKANE DEHALOGENASE; STATISTICAL POTENTIALS; PATHWAYS; BIOCATALYSTS; PREDICTION; FAMILIES

Abstract

V originále

There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiplepoint thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.

Links

GA16-06096S, research and development project

Name: Objasnění významu dynamických tunelů pro enzymatickou katalýzu: simulace a fluorescenční experimenty

Investor: Czech Science Foundation

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MUSIL, Miloš, Jan ŠTOURAČ, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP, Jaroslav ZENDULKA, Tomáš MARTÍNEK, David BEDNÁŘ and Jiří DAMBORSKÝ. FireProt: Web Server for Automated Design of Thermostable Proteins. Nucleic Acids Research. Oxford: Oxford University Press, 2017, vol. 45, W1, p. "W393"-"W399", 7 pp. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkx285.

@article{1403851,
   author = {Musil, Miloš and Štourač, Jan and Bendl, Jaroslav and Brezovský, Jan and Prokop, Zbyněk and Zendulka, Jaroslav and Martínek, Tomáš and Bednář, David and Damborský, Jiří},
   article_location = {Oxford},
   article_number = {W1},
   doi = {http://dx.doi.org/10.1093/nar/gkx285},
   keywords = {MULTIPLE SEQUENCE ALIGNMENTS; CORRELATED MUTATIONS; STABILITY CHANGES; EVOLUTIONARY CONSERVATION; HALOALKANE DEHALOGENASE; STATISTICAL POTENTIALS; PATHWAYS; BIOCATALYSTS; PREDICTION; FAMILIES},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {FireProt: Web Server for Automated Design of Thermostable Proteins},
   url = {https://loschmidt.chemi.muni.cz/peg/publications/fireprot-web-server-for-automated-design-of-thermostable-proteins/},
   volume = {45},
   year = {2017}
}

TY  - JOUR
ID  - 1403851
AU  - Musil, Miloš - Štourač, Jan - Bendl, Jaroslav - Brezovský, Jan - Prokop, Zbyněk - Zendulka, Jaroslav - Martínek, Tomáš - Bednář, David - Damborský, Jiří
PY  - 2017
TI  - FireProt: Web Server for Automated Design of Thermostable Proteins
JF  - Nucleic Acids Research
VL  - 45
IS  - W1
SP  - "W393"-"W399"
EP  - "W393"-"W399"
PB  - Oxford University Press
SN  - 03051048
KW  - MULTIPLE SEQUENCE ALIGNMENTS
KW  - CORRELATED MUTATIONS
KW  - STABILITY CHANGES
KW  - EVOLUTIONARY CONSERVATION
KW  - HALOALKANE DEHALOGENASE
KW  - STATISTICAL POTENTIALS
KW  - PATHWAYS
KW  - BIOCATALYSTS
KW  - PREDICTION
KW  - FAMILIES
UR  - https://loschmidt.chemi.muni.cz/peg/publications/fireprot-web-server-for-automated-design-of-thermostable-proteins/
N2  - There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiplepoint thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.
ER  -

MUSIL, Miloš, Jan ŠTOURAČ, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP, Jaroslav ZENDULKA, Tomáš MARTÍNEK, David BEDNÁŘ and Jiří DAMBORSKÝ. FireProt: Web Server for Automated Design of Thermostable Proteins. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2017, vol.~45, W1, p.~''W393''-''W399'', 7 pp. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkx285.

Detailed Information on Publication Record