Mapping of the spatial distribution of silver nanoparticles in
root tissues of Vicia faba by laser-induced breakdown
spectroscopy (LIBS)

J 2017

Mapping of the spatial distribution of silver nanoparticles in root tissues of Vicia faba by laser-induced breakdown spectroscopy (LIBS)

KRAJCAROVÁ, Lucie, Karel NOVOTNÝ, Marie KUMMEROVÁ, Jaroslava DUBOVÁ, Vít GLOSER et. al.

Basic information

Original name

Mapping of the spatial distribution of silver nanoparticles in root tissues of Vicia faba by laser-induced breakdown spectroscopy (LIBS)

Authors

KRAJCAROVÁ, Lucie (203 Czech Republic, belonging to the institution), Karel NOVOTNÝ (203 Czech Republic, guarantor, belonging to the institution), Marie KUMMEROVÁ (203 Czech Republic, belonging to the institution), Jaroslava DUBOVÁ (203 Czech Republic, belonging to the institution), Vít GLOSER (203 Czech Republic, belonging to the institution) and Jozef KAISER (203 Czech Republic)

Edition

Talanta, AMSTERDAM, NETHERLANDS, ELSEVIER SCIENCE BV, 2017, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.244

RIV identification code

RIV/00216224:14310/17:00099504

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.talanta.2017.05.055

UT WoS

000404491200005

Keywords (in Czech)

Spektroskopie laserem buzeného plazmatu; Stříbrné nanočástice; Distribuce; Kořenová tkáň

Keywords in English

Laser-induced breakdown spectroscopy; Silver nanoparticles; Distribution; Root tissues

Abstract

V originále

The manuscript presents a procedure for optimal sample preparation and the mapping of the spatial distribution of metal ions and nanoparticles in plant roots using laser-induced breakdown spectroscopy (LIBS) in a double-pulse configuration (DP LIBS) in orthogonal reheating mode. Two Nd:YAG lasers were used; the first one was an ablation laser (UP-266 MACRO, New Wave, USA) with a wavelength of 266 nm, and the second one (Brilliant, Quantel, France), with a fundamental wavelength of 1064 nm, was used to reheat the microplasma. Seedlings of Vicia faba were cultivated for 7 days in CuSO4 or AgNO3 solutions with a concentration of 10 mu mol 1(-1) or in a solution of silver nanoparticles (AgNPs) with a concentration of 10 mu mol 1(-1) of total Ag, and in distilled water as a control. The total contents of the examined metals in the roots after sample mineralization as well as changes.in the concentrations of the metals in the cultivation solutions were monitored by ICP-OES. Root samples embedded in the TissueTek medium and cut into 40 gm thick cross sections using the Cryo-Cut Microtome proved to be best suited for an accurate LIBS analysis with a 50 gm spatial resolution. 2D raster maps of elemental distribution were created for the emission lines of Cu(I) at 324.754 nm and Ag(I) at 328.068 nm. The limits of detection of DP LIES for the root cross sections were estimated to be 4 pg for Cu, 18 pg for Ag, and 3 pg for AgNPs. The results of Ag spatial distribution mapping indicated that unlike Ag+ ions, AgNPs do not penetrate into the inner tissues of Vicia faba roots but stay in their outermost layers. The content of Ag in roots cultivated in the AgNP solution was one order of magnitude lower compared to roots cultivated in the metal ion solutions. The significantly smaller concentration of Ag in root tissues cultivated in the AgNP solution also supports the conclusion that the absorption and uptake of AgNPs by roots of Vicia faba is very slow.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

KRAJCAROVÁ, Lucie, Karel NOVOTNÝ, Marie KUMMEROVÁ, Jaroslava DUBOVÁ, Vít GLOSER and Jozef KAISER. Mapping of the spatial distribution of silver nanoparticles in root tissues of Vicia faba by laser-induced breakdown spectroscopy (LIBS). Talanta. AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV, 2017, vol. 173, October, p. 28-35. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2017.05.055.

@article{1404203,
   author = {Krajcarová, Lucie and Novotný, Karel and Kummerová, Marie and Dubová, Jaroslava and Gloser, Vít and Kaiser, Jozef},
   article_location = {AMSTERDAM, NETHERLANDS},
   article_number = {October},
   doi = {http://dx.doi.org/10.1016/j.talanta.2017.05.055},
   keywords = {Laser-induced breakdown spectroscopy; Silver nanoparticles; Distribution; Root tissues},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Mapping of the spatial distribution of silver nanoparticles in root tissues of Vicia faba by laser-induced breakdown spectroscopy (LIBS)},
   volume = {173},
   year = {2017}
}

TY  - JOUR
ID  - 1404203
AU  - Krajcarová, Lucie - Novotný, Karel - Kummerová, Marie - Dubová, Jaroslava - Gloser, Vít - Kaiser, Jozef
PY  - 2017
TI  - Mapping of the spatial distribution of silver nanoparticles in root tissues of Vicia faba by laser-induced breakdown spectroscopy (LIBS)
JF  - Talanta
VL  - 173
IS  - October
SP  - 28-35
EP  - 28-35
PB  - ELSEVIER SCIENCE BV
SN  - 00399140
KW  - Laser-induced breakdown spectroscopy
KW  - Silver nanoparticles
KW  - Distribution
KW  - Root tissues
N2  - The manuscript presents a procedure for optimal sample preparation and the mapping of the spatial distribution of metal ions and nanoparticles in plant roots using laser-induced breakdown spectroscopy (LIBS) in a double-pulse configuration (DP LIBS) in orthogonal reheating mode. Two Nd:YAG lasers were used; the first one was an ablation laser (UP-266 MACRO, New Wave, USA) with a wavelength of 266 nm, and the second one (Brilliant, Quantel, France), with a fundamental wavelength of 1064 nm, was used to reheat the microplasma. Seedlings of Vicia faba were cultivated for 7 days in CuSO4 or AgNO3 solutions with a concentration of 10 mu mol 1(-1) or in a solution of silver nanoparticles (AgNPs) with a concentration of 10 mu mol 1(-1) of total Ag, and in distilled water as a control. The total contents of the examined metals in the roots after sample mineralization as well as changes.in the concentrations of the metals in the cultivation solutions were monitored by ICP-OES. Root samples embedded in the TissueTek medium and cut into 40 gm thick cross sections using the Cryo-Cut Microtome proved to be best suited for an accurate LIBS analysis with a 50 gm spatial resolution. 2D raster maps of elemental distribution were created for the emission lines of Cu(I) at 324.754 nm and Ag(I) at 328.068 nm. The limits of detection of DP LIES for the root cross sections were estimated to be 4 pg for Cu, 18 pg for Ag, and 3 pg for AgNPs. The results of Ag spatial distribution mapping indicated that unlike Ag+ ions, AgNPs do not penetrate into the inner tissues of Vicia faba roots but stay in their outermost layers. The content of Ag in roots cultivated in the AgNP solution was one order of magnitude lower compared to roots cultivated in the metal ion solutions. The significantly smaller concentration of Ag in root tissues cultivated in the AgNP solution also supports the conclusion that the absorption and uptake of AgNPs by roots of Vicia faba is very slow.
ER  -

KRAJCAROVÁ, Lucie, Karel NOVOTNÝ, Marie KUMMEROVÁ, Jaroslava DUBOVÁ, Vít GLOSER and Jozef KAISER. Mapping of the spatial distribution of silver nanoparticles in root tissues of Vicia faba by laser-induced breakdown spectroscopy (LIBS). \textit{Talanta}. AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV, 2017, vol.~173, October, p.~28-35. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2017.05.055.

Detailed Information on Publication Record