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@article{1404252,
author = {Kotásková, Iva and Mališová, Barbora and Obručová, Hana and Holá, Veronika and Peroutková, Tereza and Růžička, Filip and Freiberger, Tomáš},
article_location = {Basel},
article_number = {6},
doi = {http://dx.doi.org/10.1159/000484524},
keywords = {Catheter; Complex sample; Mixed chromatogram; PCR denaturing gradient gel electrophoresis; RipSeq Mixed},
language = {eng},
issn = {1464-1801},
journal = {Journal of Molecular Microbiology and Biotechnology},
title = {Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis},
volume = {27},
year = {2017}
}
TY - JOUR
ID - 1404252
AU - Kotásková, Iva - Mališová, Barbora - Obručová, Hana - Holá, Veronika - Peroutková, Tereza - Růžička, Filip - Freiberger, Tomáš
PY - 2017
TI - Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
JF - Journal of Molecular Microbiology and Biotechnology
VL - 27
IS - 6
SP - 350-355
EP - 350-355
PB - Karger
SN - 14641801
KW - Catheter
KW - Complex sample
KW - Mixed chromatogram
KW - PCR denaturing gradient gel electrophoresis
KW - RipSeq Mixed
N2 - Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment.
ER -
KOTÁSKOVÁ, Iva, Barbora MALIŠOVÁ, Hana OBRUČOVÁ, Veronika HOLÁ, Tereza PEROUTKOVÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis. \textit{Journal of Molecular Microbiology and Biotechnology}. Basel: Karger, 2017, vol.~27, No~6, p.~350-355. ISSN~1464-1801. Available from: https://dx.doi.org/10.1159/000484524.
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