Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum

KYSEĽ, Peter, Nikola KOSTLÁNOVÁ, Josef HOUSER, Eva DUBSKÁ, Ondřej ŠULÁK and Michaela WIMMEROVÁ. Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum. In XVIII. setkání biochemiků a molekulárních biologů. 2017. ISBN 978-80-210-8765-1.

Basic information

• Original name: Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum
• Authors: KYSEĽ, Peter (703 Slovakia, belonging to the institution), Nikola KOSTLÁNOVÁ (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Eva DUBSKÁ (203 Czech Republic, belonging to the institution), Ondřej ŠULÁK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: XVIII. setkání biochemiků a molekulárních biologů, 2017.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14740/17:00099517
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-210-8765-1
• Keywords in English: Ralstonia; phytopathogen; lectin; trimer; protein purification; SDS-PAGE; protein stability; nano differential scanning fluorimetry; isothermal titration calorimetry; analytical ultracentrifugation; protein crystallization; X-ray diffraction
• Tags: rivok

Abstract

Lectin RS20L from phytopathogen Ralstonia solanacearum showed a low solubility without a sugar ligand. A selected panel of additives was tested using the nano differential scanning fluorimetry (nanoDSF). The manganese chloride together with higher pH (8.1) has shown to dramaticaly increase the stability important for crystallization.of RS20L protein. Crystallization screening provided crystals of RS20L in conditions of 0.1 M Hepes pH=7.5 with 10% (v/v) polyethylene glycol of different molecular weight. Increasing the temperature dramaticaly improved the reproducibility of crystallization.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR