Crystallization and stability studies of trimeric lectin RS20L
from phytopathogen Ralstonia solanacearum

a 2017

Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum

KYSEĽ, Peter, Nikola KOSTLÁNOVÁ, Josef HOUSER, Eva DUBSKÁ, Ondřej ŠULÁK et. al.

Basic information

Original name

Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum

Authors

KYSEĽ, Peter (703 Slovakia, belonging to the institution), Nikola KOSTLÁNOVÁ (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Eva DUBSKÁ (203 Czech Republic, belonging to the institution), Ondřej ŠULÁK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

XVIII. setkání biochemiků a molekulárních biologů, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14740/17:00099517

Organization unit

Central European Institute of Technology

ISBN

978-80-210-8765-1

Keywords in English

Ralstonia; phytopathogen; lectin; trimer; protein purification; SDS-PAGE; protein stability; nano differential scanning fluorimetry; isothermal titration calorimetry; analytical ultracentrifugation; protein crystallization; X-ray diffraction

Abstract

V originále

Lectin RS20L from phytopathogen Ralstonia solanacearum showed a low solubility without a sugar ligand. A selected panel of additives was tested using the nano differential scanning fluorimetry (nanoDSF). The manganese chloride together with higher pH (8.1) has shown to dramaticaly increase the stability important for crystallization.of RS20L protein. Crystallization screening provided crystals of RS20L in conditions of 0.1 M Hepes pH=7.5 with 10% (v/v) polyethylene glycol of different molecular weight. Increasing the temperature dramaticaly improved the reproducibility of crystallization.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

KYSEĽ, Peter, Nikola KOSTLÁNOVÁ, Josef HOUSER, Eva DUBSKÁ, Ondřej ŠULÁK and Michaela WIMMEROVÁ. Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum. In XVIII. setkání biochemiků a molekulárních biologů. 2017. ISBN 978-80-210-8765-1.

@proceedings{1404306,
   author = {Kyseľ, Peter and Kostlánová, Nikola and Houser, Josef and Dubská, Eva and Šulák, Ondřej and Wimmerová, Michaela},
   booktitle = {XVIII. setkání biochemiků a molekulárních biologů},
   keywords = {Ralstonia; phytopathogen; lectin; trimer; protein purification; SDS-PAGE; protein stability; nano differential scanning fluorimetry; isothermal titration calorimetry; analytical ultracentrifugation; protein crystallization; X-ray diffraction},
   language = {eng},
   isbn = {978-80-210-8765-1},
   title = {Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum},
   year = {2017}
}

TY  - CONF
ID  - 1404306
AU  - Kyseľ, Peter - Kostlánová, Nikola - Houser, Josef - Dubská, Eva - Šulák, Ondřej - Wimmerová, Michaela
PY  - 2017
TI  - Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum
SN  - 9788021087651
KW  - Ralstonia
KW  - phytopathogen
KW  - lectin
KW  - trimer
KW  - protein purification
KW  - SDS-PAGE
KW  - protein stability
KW  - nano differential scanning fluorimetry
KW  - isothermal titration calorimetry
KW  - analytical ultracentrifugation
KW  - protein crystallization
KW  - X-ray diffraction
N2  - Lectin RS20L from phytopathogen Ralstonia solanacearum showed a low solubility without a sugar ligand. A selected panel of additives was tested using the nano differential scanning fluorimetry (nanoDSF). The manganese chloride together with higher pH (8.1) has shown to dramaticaly increase the stability important for crystallization.of RS20L protein. Crystallization screening provided crystals of RS20L in conditions of 0.1 M Hepes pH=7.5 with 10% (v/v) polyethylene glycol of different molecular weight. Increasing the temperature dramaticaly improved the reproducibility of crystallization.
ER  -

KYSEĽ, Peter, Nikola KOSTLÁNOVÁ, Josef HOUSER, Eva DUBSKÁ, Ondřej ŠULÁK and Michaela WIMMEROVÁ. Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum. In \textit{XVIII. setkání biochemiků a molekulárních biologů}. 2017. ISBN~978-80-210-8765-1.

Detailed Information on Publication Record